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| {{MitoPedia
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| |abbr=PM+S+Rot_OXPHOS
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| |description=[[File:1PM;2D;3G;4S;5Rot;6Omy;7U-.png|400px]]
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| |info='''A: simultaneous determination of O2 flux and the redox state of the Q-pool'''
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| }}
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| ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS
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| ::: '''[[SUIT protocol pattern]]:''' orthogonal 1PM;2D;3S;4Rot;5U;-
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| This is a protocol to investigate simultanouesly oxygen flux and the redox state of the Q-pool in the [[NADH Electron transfer-pathway state| N-]] and [[NS-pathway control state| NS-pathway]] control state on isolated mitochondria and permeabilized cells.
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| __TOC__
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| Communicated by [[Komlodi T]] (names to be added) (last update 2021-02-01)
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| == Specific SUIT protocols ==
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| === SUIT-031 Q mt D072 ===
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| [[File:1PM;2D;2S;4Rot;5U;6Ama.png|300px]]
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| [[File:SUIT-031 Q mt D072 O2.png|600px]]
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| [[File:SUIT-031 Q mt D072 Q.png|600px]]
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| :::* [[SUIT-031 Q mt D072]] simultaneous determination of O<sub>2</sub> flux and the redox ratio of the Q-pool in isolated mitochondria.
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| === SUIT-031 Q ce-pce D074 ===
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| [[File:ce1;1Dig;1PM;2D;2S;4Rot;5U;6Ama.png|300px]]
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| [[File:SUIT-031 Q ce-pce D074 O2.png|600px]]
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| [[File:SUIT-031 Q ce-pce D074 Q.png|600px]]
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| :::* [[SUIT-031 Q ce-pce D074]] simultaneous determination of O<sub>2</sub> flux and the redox ratio of the Q-pool in permeabilized cells.
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| === SUIT-031 O2 mt D075 ===
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| [[File:1PM;2D;2S;4Rot;5U;6Ama.png|300px]]
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| [[File:SUIT-031 O2 mt D075.png|600px]]
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| {{Template:SUIT-031}}
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| == Strengths and limitations ==
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| :::* NS-OXPHOS capacity provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity.
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| :::* A succinate concentration of >10 mM may be required for saturating SE capacity.
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| :::+ You obtain information in a single protocol about the [[NS-pathway control state| NS-]], [[Succinate pathway control state| S]] and [[NADH Electron transfer-pathway state| N-]] pathway control state.
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| :::- CIV activity cannot be determined and Cytochrome c test cannot be performed together with the fluorescence dyes.
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| :::- Careful washing is required after the experiment to avoid carry-over of the inhibitors and uncoupler.
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| == Compare SUIT protocols ==
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| :::*
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| == References ==
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| {{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-031]]
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| |?Was published in year=Year
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| |?Has title=Reference
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| |?Mammal and model=Organism
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| |?Tissue and cell=Tissue;cell
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| |format=broadtable
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| |limit=5000
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| |offset=0
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| |sort=Was published in year
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| |order=descending
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| }}
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| {{#ask:[[Category:Abstracts]] [[Additional label::SUIT-031]] [[Instrument and method::O2k-Protocol]]
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| |?Was submitted in year=Year
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| |?Has title=Reference
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| |?Mammal and model=Organism
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| |?Tissue and cell=Tissue;cell
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| |format=broadtable
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| |limit=5000
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| |offset=0
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| |sort=Was published in year
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| |order=descending
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| }}
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| {{MitoPedia concepts
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| |mitopedia concept=MiP concept, SUIT protocol, Recommended
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| }}
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| {{MitoPedia methods
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| |mitopedia method=Respirometry,
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| }}
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