SUIT-022

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MitoPedia

SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf »Versions


O2k-Application: O2

SUIT-category:
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both SUIT-022 O2 ce D051 and SUIT-023 O2 ce D053 must be carried out in parallel.

Communicated by Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-04-04)

Specific SUIT protocols

SUIT-022 O2 ce D051

Ce1;ce2KCN;ce3SHAM.png D051 O2 traces.png

SUIT-022 O2 ce D00SHAM

Ce1;ce2SHAM;ce3KCN.png D053 O2 traces.png


Strengths and limitations

  • SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to SUIT-023.
+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry