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SUIT-022

From Bioblast


high-resolution terminology - matching measurements at high-resolution


SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf Β»Versions


O2k-Application: O2

SUIT-category:
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00## must be carried out in paralel.

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)

Specific SUIT protocols

SUIT-022 O2 ce D00#

Ce1;ce2KCN;ce3SHAM.png 400px

SUIT-022 O2 ce D00##

Ce1;ce2SHAM;ce3KCN.png 400px


Strengths and limitations

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry