Difference between revisions of "SUIT-022"
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::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ||
The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D051]] and [[SUIT-023 O2 ce D053]] must be carried out in parallel. | The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both [[SUIT-022 O2 ce D051]] and [[SUIT-023 O2 ce D053]] must be carried out in parallel. | ||
__TOC__ | __TOC__ | ||
Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-04) | Communicated by [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-04) | ||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]] | :::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to [[SUIT-023]]. | ||
:::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised. | :::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised. | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
Revision as of 10:57, 9 April 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-022
Description
Reference: A: Determination of the respiration due to the alternative oxidase pathway 
»Versions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both SUIT-022 O2 ce D051 and SUIT-023 O2 ce D053 must be carried out in parallel.
Communicated by Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-04-04)
Specific SUIT protocols
SUIT-022 O2 ce D051
- SUIT-022 O2 ce D051 for intact microalgal cells (ce)
SUIT-022 O2 ce D00SHAM
- SUIT-023 O2 ce D053 for for intact microalgal cells (ce)
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the contribution of the cytochrome c pathway to respiration, it must be carried out in parallel to SUIT-023.
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry
