Difference between revisions of "SUIT-022"

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::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
 
::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
  
The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[alternative oxidase]] pathway, which is [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome ''c'' pathway (see [[SUIT-023]]).  
+
The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the [[alternative oxidase]] pathway, which is [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome ''c'' oxidase pathway (see [[SUIT-023]]).  
  
  
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*  [[SUIT-022 O2 ce D051]] for intact microalgal cells (ce)
 
*  [[SUIT-022 O2 ce D051]] for intact microalgal cells (ce)
  
{{Template:SUIT-022}}
+
{{Template:SUIT-022 O2 ce D00}}
  
 
== Strengths and limitations ==
 
== Strengths and limitations ==

Revision as of 11:34, 9 April 2019

Bioblasts - Richard Altmann and MiPArt by Odra Noel
MitoPedia         Terms and abbreviations         Concepts and methods         MitoPedia: SUIT         MiP and biochemistry         Preprints and history



MitoPedia

SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf »Versions


O2k-Application: O2

SUIT-category: ce
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c oxidase pathway (see SUIT-023).


Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-022 O2 ce D051

Ce1;ce2KCN;ce3SHAM.png D051 O2 traces.png

MitoPedia: SUIT

Steps and respiratory states

Ce1;ce2KCN;ce3SHAM.png Ce1;ce2KCN;ce3SHAM.v2.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2KCN ROX or ROUTINE ce1;ce2KCN
ce3SHAM ROX ce1;ce2KCN;ce3SHAM


Questions.jpg


Click to expand or collaps

Strengths and limitations

  • SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome c pathway, it must be carried out in parallel to SUIT-023.
+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry