Difference between revisions of "SUIT-022"

From Bioblast
Jump to: navigation, search
Line 24: Line 24:
  
 
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome ''c'' pathway, it must be carried out in parallel to [[SUIT-023]].
 
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome ''c'' pathway, it must be carried out in parallel to [[SUIT-023]].
:::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
+
:::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
 
:::+ Short duration of experiment
 
:::+ Short duration of experiment
  

Revision as of 11:21, 9 April 2019

Bioblasts - Richard Altmann and MiPArt by Odra Noel
MitoPedia         Terms and abbreviations         Concepts and methods         MitoPedia: SUIT         MiP and biochemistry         Preprints and history



MitoPedia

SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf »Versions


O2k-Application: O2

SUIT-category: ce
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023).


Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-022 O2 ce D051

Ce1;ce2KCN;ce3SHAM.png D051 O2 traces.png


Strengths and limitations

  • SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome c pathway, it must be carried out in parallel to SUIT-023.
+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry