Difference between revisions of "SUIT-022"

Revision as of 10:28, 9 April 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway »Versions

O2k-Application: O2

SUIT-category:

SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D051 and SUIT-023 O2 ce D053 must be carried out in parallel.

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)

Specific SUIT protocols

SUIT-022 O2 ce D051

SUIT-022 O2 ce D051 for intact microalgal cells (ce)

SUIT-022 O2 ce D00SHAM

SUIT-023 O2 ce D053 for for intact microalgal cells (ce)

Strengths and limitations

SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

@@ Line 6: / Line 6: @@
 ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
-The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D051]] and [[SUIT-022 O2 ce D053]] must be carried out in paralel.
+The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D051]] and [[SUIT-023 O2 ce D053]] must be carried out in parallel.
 __TOC__
@@ Line 14: / Line 14: @@
 === SUIT-022 O2 ce D051 ===
 [[File:ce1;ce2KCN;ce3SHAM.png|400px]]
-[[File:D00#_O2_traces.png|400px]]
+[[File:D051_O2_traces.png|400px]]
-*  [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce)
+*  [[SUIT-022 O2 ce D051]] for intact microalgal cells (ce)
 === SUIT-022 O2 ce D00SHAM ===
 [[File:ce1;ce2SHAM;ce3KCN.png|400px]]
-[[File:D00SHAM_O2_traces.png|400px]]
+[[File:D053_O2_traces.png|400px]]
-*  [[SUIT-022 O2 ce D00SHAM]] for for intact microalgal cells (ce)
+*  [[SUIT-023 O2 ce D053]] for for intact microalgal cells (ce)
 {{Template:SUIT-022}}
@@ Line 26: / Line 26: @@
 == Strengths and limitations ==
-:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]
+:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on cells on [[ROUTINE]]
 :::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
 :::+ Short duration of experiment