Difference between revisions of "SUIT-022"
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::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ||
The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce | The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00SHAM]] must be carried out in paralel. Β | ||
__TOC__ | __TOC__ | ||
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*Β [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce) | *Β [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce) | ||
=== SUIT-022 O2 ce | === SUIT-022 O2 ce D00SHAM === | ||
[[File:ce1;ce2SHAM;ce3KCN.png|400px]] | [[File:ce1;ce2SHAM;ce3KCN.png|400px]] | ||
[[File: | [[File:D00SHAM_O2_traces.png|400px]] | ||
*Β [[SUIT-022 O2 ce | *Β [[SUIT-022 O2 ce D00SHAM]] for for intact microalgal cells (ce) | ||
{{Template:SUIT-022}} | {{Template:SUIT-022}} |
Revision as of 17:25, 4 April 2019
Description
Reference: A: Determination of the respiration due to the alternative oxidase pathway Β»Versions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00SHAM must be carried out in paralel.
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)
Specific SUIT protocols
SUIT-022 O2 ce D00#
- SUIT-022 O2 ce D00# for intact microalgal cells (ce)
SUIT-022 O2 ce D00SHAM
- SUIT-022 O2 ce D00SHAM for for intact microalgal cells (ce)
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry