Difference between revisions of "SUIT-022"

Revision as of 17:24, 4 April 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway »Versions

O2k-Application: O2

SUIT-category:

SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00# must be carried out in paralel.

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)

Specific SUIT protocols

SUIT-022 O2 ce D00#

400px

SUIT-022 O2 ce D00# for intact microalgal cells (ce)

SUIT-022 O2 ce D00##

400px

SUIT-022 O2 ce D00## for for intact microalgal cells (ce)

Strengths and limitations

SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

@@ Line 6: / Line 6: @@
 ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
-The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00#]] must be carried out simultaneously.
+The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00#]] must be carried out in paralel.
 __TOC__
@@ Line 17: / Line 17: @@
 *  [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce)
-=== SUIT-022 O2 ce D00# ===
+=== SUIT-022 O2 ce D00## ===
 [[File:ce1;ce2SHAM;ce3KCN.png|400px]]
-[[File:D00#_O2_traces.png|400px]]
+[[File:D00##_O2_traces.png|400px]]
-*  [[SUIT-022 O2 ce D00#]] for for intact microalgal cells (ce)
+*  [[SUIT-022 O2 ce D00##]] for for intact microalgal cells (ce)
 {{Template:SUIT-022}}