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Difference between revisions of "SUIT-022"

From Bioblast
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{{MitoPedia
{{MitoPedia
|info='''A: AOX'''
|info='''A:''' Determination of the respiration due to the alternative oxidase pathway [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-022.pdf|Bioblast pdf]] Β»[http://wiki.oroboros.at/index.php/File:SUIT-022.pdf Versions]
|application=O2
|application=O2
}}
}}
::: '''[[Categories of SUIT protocols |SUIT-category]]:'''
::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00#]] must be carried out simultaneously.
__TOC__
Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-04)
== Specific SUIT protocols ==
=== SUIT-022 O2 ce D00# ===
[[File:ce1;ce2KCN;ce3SHAM.png|400px]]
[[File:D00#_O2_traces.png|400px]]
*Β  [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce)
=== SUIT-022 O2 ce D00# ===
[[File:ce1;ce2SHAM;ce3KCN.png|400px]]
[[File:D00#_O2_traces.png|400px]]
*Β  [[SUIT-022 O2 ce D00#]] for for intact microalgal cells (ce)
{{Template:SUIT-022}}
== Strengths and limitations ==
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]
:::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
:::+ Short duration of experiment
:::- Estimation of the linear coupling control is not done
:::- The use of [[cyanide| cyanide]] requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
== Compare SUIT protocols ==
== References ==
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-022]]
|?Was published in year=Year
|?Has title=Reference
|?Mammal and model=Organism
|?Tissue and cell=Tissue;cell
|format=broadtable
|limit=5000
|offset=0
|sort=Was published in year
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{{MitoPedia concepts
{{MitoPedia concepts
|mitopedia concept=MiP concept, SUIT protocol, Recommended
|mitopedia concept=MiP concept, SUIT protocol, Recommended

Revision as of 15:52, 4 April 2019


high-resolution terminology - matching measurements at high-resolution


SUIT-022

Description

Reference: A: Determination of the respiration due to the alternative oxidase pathway Bioblast pdf Β»Versions


O2k-Application: O2

SUIT-category:
SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00# must be carried out simultaneously.

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)

Specific SUIT protocols

SUIT-022 O2 ce D00#

Ce1;ce2KCN;ce3SHAM.png 400px

SUIT-022 O2 ce D00#

Ce1;ce2SHAM;ce3KCN.png 400px


Strengths and limitations

+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
+ Short duration of experiment
- Estimation of the linear coupling control is not done
- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended 


MitoPedia methods: Respirometry