Difference between revisions of "SUIT-022"
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{{MitoPedia | {{MitoPedia | ||
|info='''A: | |info='''A:''' Determination of the respiration due to the alternative oxidase pathway [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-022.pdf|Bioblast pdf]] Β»[http://wiki.oroboros.at/index.php/File:SUIT-022.pdf Versions] | ||
|application=O2 | |application=O2 | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | |||
::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | |||
The SUIT-022 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]]. To obtain a complete estimation, both [[SUIT-022 O2 ce D00#]] and [[SUIT-022 O2 ce D00#]] must be carried out simultaneously. | |||
__TOC__ | |||
Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-04) | |||
== Specific SUIT protocols == | |||
=== SUIT-022 O2 ce D00# === | |||
[[File:ce1;ce2KCN;ce3SHAM.png|400px]] | |||
[[File:D00#_O2_traces.png|400px]] | |||
*Β [[SUIT-022 O2 ce D00#]] for intact microalgal cells (ce) | |||
=== SUIT-022 O2 ce D00# === | |||
[[File:ce1;ce2SHAM;ce3KCN.png|400px]] | |||
[[File:D00#_O2_traces.png|400px]] | |||
*Β [[SUIT-022 O2 ce D00#]] for for intact microalgal cells (ce) | |||
{{Template:SUIT-022}} | |||
== Strengths and limitations == | |||
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal cells]] on [[ROUTINE]] | |||
:::+ This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised. | |||
:::+ Short duration of experiment | |||
:::- Estimation of the linear coupling control is not done | |||
:::- The use of [[cyanide| cyanide]] requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety. | |||
== Compare SUIT protocols == | |||
== References == | |||
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Protocol]] [[Additional label::SUIT-022]] | |||
|?Was published in year=Year | |||
|?Has title=Reference | |||
|?Mammal and model=Organism | |||
|?Tissue and cell=Tissue;cell | |||
|format=broadtable | |||
|limit=5000 | |||
|offset=0 | |||
|sort=Was published in year | |||
|order=descending | |||
}} | |||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=MiP concept, SUIT protocol, Recommended | |mitopedia concept=MiP concept, SUIT protocol, Recommended |
Revision as of 15:52, 4 April 2019
Description
Reference: A: Determination of the respiration due to the alternative oxidase pathway Β»Versions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE. To obtain a complete estimation, both SUIT-022 O2 ce D00# and SUIT-022 O2 ce D00# must be carried out simultaneously.
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-04)
Specific SUIT protocols
SUIT-022 O2 ce D00#
- SUIT-022 O2 ce D00# for intact microalgal cells (ce)
SUIT-022 O2 ce D00#
- SUIT-022 O2 ce D00# for for intact microalgal cells (ce)
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells on ROUTINE
- + This protocol can be used with other type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry