View source for SF6847

{{MitoPedia
|abbr=SF6847
|description='''SF6847''' (C<sub>18</sub>H<sub>22</sub>N<sub>2O</sub>), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent [[uncoupler]] of [[oxidative phosphorylation]], being used in the nM range. Like all uncouplers, SF6847 concentrations must be titrated carefully to evaluate the [[Uncoupler#Optimum_uncoupler_concentration|optimum concentration]] for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.
|info=[[Chinopoulos 2014 Methods Enzymol]], [https://www.ncbi.nlm.nih.gov/pubmed/6498178 Terada 1984 Biochim Biophys Acta]
}}
== Application in [[HRR]] ==
{{Chemical_description
|abbr=SF6847
|trivial name=Tyrphostin A9 or Malonoben
|complete name=3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenylmethylenepropanedinitrile
|chem formula=C<sub>18</sub>H<sub>22</sub>N<sub>2</sub>O
|molar mass=282.38
|vendor=Sigma-Aldrich
|product number=T182
|store at=RT
|sensitivity=
|cas=10537-47-0
|h statements=H301, H311
|h info=toxic if swallowed, toxic in contact with skin
}}<!--:::: '''SF 6847'''/tyrphostin A9/malonoben (C<sub>18</sub>H<sub>22</sub>N<sub>2</sub>O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: M = 282.38 g·mol<sup>-1</sup>. Alternative source: Enzo, BML-EI215.
:::: <span style="color:#8B008B"> '''Caution:''' Toxic!</span>-->


::: '''Preparation of 25 mM stock solution''' (dissolved in ethanol abs.):
::::# Weigh 5 mg in a dark glass vial, dissolve in 708.3 µL ethanol. 
::::# Store in dark glass vials at -20 °C.


::: '''Preparation of 50 µM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''':
::::# Mix 998 µL ethanol abs. and 2 µL of the 25 mM stock solution.
::::# Divide into 0.1 ml aliquotes, use dark glass vials.
::::# Store at -20 °C.

::: » '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL steps of 50 µM stock solution using a 10 µL Hamilton syringe.
::::* Final concentration: 25 nM steps.


::: '''Preparation of 12.5 µM stock solution''' (dissolved in ethanol abs.)  for '''0.5-mL O2k-chamber''':
::::# Add 300 µL ethanol abs. and 100 µL of the 50 µM stock solution.
::::# Divide into 0.1 ml aliquots in dark glass vials. Store at -20 °C.

::: » '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL of 12.5 µM stock solution using a 10 µL Hamilton syringe.
::::* Final concentration: 25 nM steps.


:::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

{{Keywords: Uncoupling}}

{{MitoPedia topics
|mitopedia topic=Uncoupler
}}