- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
Rotenone is an (synonym. 2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on) inhibitor of complex I via inhibition of NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Rot: Rotenone (Rotenone ≥ 95%, C23H22O6); Sigma R 8875, 1 g, store at R.T.; FW = 394.42
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- 2. Divide into 100 µl aliquots (use dark glass vials).
- 3) Store frozen at -20 °C.
Oxygraph-2k manual titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe.
- Final conc. in 2 ml O2k-Chamber: 0.5 µM.
