- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
(2R,6aS,12aS)-1,2,6,6a,12,12a-hexahydro-2-isopropenyl-8,9 dimethoxychromeno[3,4-b]furo(2,3-h)chromen-6-on; C23H22O6. Inhibitor of complex I via inhibition of NADH oxidation.It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: MiPNet11.09, MiPNet09.12, MiPNet12.15, MiPNet03.02
MitoPedia topics:
Inhibitor
Application in HRR
Rot: Rotenone (Rotenone ≥ 95%, C23H22O6); Sigma R 8875, 1 g, store at R.T.: FW = 394.42)
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!
Preparation of 1.0 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 0.39 mg of Rotenone and dissolve in 1 ml of absolute ethanol
- 2) divide into 100 µl aliquots (use dark glass vials)
- 3) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
