Difference between revisions of "Rotenone"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Rot | |abbr=Rot | ||
|description='''Rotenone''' is an inhibitor of [[Complex I]] and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in | |description='''Rotenone''' is an inhibitor of [[Complex I]] (C<sub>I</sub>) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in C<sub>I</sub> to ubiquinone via binding to the ubiquinone binding site of C<sub>I</sub>. See also [[S(Rot)|S<sub>(Rot)</sub>]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]] | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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== Inhibition of | == Inhibition of C<sub>I</sub> by rotenone == | ||
* Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ >>PMC2724837] | * Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ >>PMC2724837] | ||
Revision as of 10:00, 24 July 2014
- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
Rotenone is an inhibitor of Complex I (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Rot: Rotenone (C23H22O6); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).
Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!.
Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):
- 1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- 2. Divide into 100 µl aliquots (use dark glass vials).
- 3) Store frozen at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
Inhibition of CI by rotenone
- Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. >>PMC2724837
