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Difference between revisions of "Rotenone"

From Bioblast
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|description='''Rotenone''' is an inhibitor of [[Complex I]] (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[Complex II-linked substrate state |Complex II-linked substrate state, S<sub>(Rot)</sub>]].
|description='''Rotenone''' is an inhibitor of [[Complex I]] (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[Complex II-linked substrate state |Complex II-linked substrate state, S<sub>(Rot)</sub>]].
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]]
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]]
}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
|mitopedia topic=Inhibitor
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==


:::: '''Rot: Rotenone''' (C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).
::::'''Rot: Rotenone''' (C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).


:::: <span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial).</span>.
:::: <span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial).</span>.
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::::* Final concentration: 0.5 µM.
::::* Final concentration: 0.5 µM.


 
'''Microalgae applications'''
:For the specific case of microalgal cells it is recommended to prepare higher concentration:
:For the specific case of microalgal cells it is recommended to prepare higher concentration:
::: '''Preparation of 5.5 mM stock solution''' (dissolved in 99.9% ethanol)
::: '''Preparation of 5.5 mM stock solution''' (dissolved in 99.9% ethanol)
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== Inhibition of CI by rotenone ==
== Inhibition of CI by rotenone ==
::::* Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787:384–92. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ >>PMC2724837]
::::* Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787:384–92. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2724837/ >>PMC2724837]
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
|mitopedia topic=Inhibitor
}}

Revision as of 15:48, 10 April 2019


high-resolution terminology - matching measurements at high-resolution


Rotenone

Description

Rotenone is an inhibitor of Complex I (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also Complex II-linked substrate state, S(Rot).

Abbreviation: Rot

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MiPNet03.02

Application in HRR

Rot: Rotenone (C23H22O6); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).
Caution: Very toxic and light sensitive (store solution in a dark glass vial)..


Preparation of 1 mM stock solution (dissolved in 99.9% ethanol)
  1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
  2. Divide into 100 µl aliquots (use dark glass vials).
  3. Store frozen at -20 °C.


O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
  • Final concentration: 0.5 µM.

Microalgae applications

For the specific case of microalgal cells it is recommended to prepare higher concentration:
Preparation of 5.5 mM stock solution (dissolved in 99.9% ethanol)
  1. Weigh 4.4 mg of rotenone and dissolve in 2 ml of ethanol absolute (99.9%).
  2. Divide into 200 µl aliquots (use dark glass vials).
  3. Store frozen at -20 °C.

Inhibition of CI by rotenone

  • Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787:384–92. >>PMC2724837






MitoPedia topics: Inhibitor