Difference between revisions of "Rotenone"
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|description='''Rotenone''' is an inhibitor of [[Complex I]] (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[Complex II-linked substrate state |Complex II-linked substrate state, S<sub>(Rot)</sub>]]. | |description='''Rotenone''' is an inhibitor of [[Complex I]] (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also [[Complex II-linked substrate state |Complex II-linked substrate state, S<sub>(Rot)</sub>]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]] | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MiPNet03.02]] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
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== Application in [[HRFR]] == | == Application in [[HRFR]] == | ||
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:::: '''Rot: Rotenone''' (C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%). | :::: '''Rot: Rotenone''' (C<sub>23</sub>H<sub>22</sub>O<sub>6</sub>); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%). | ||
:::: <span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial). | :::: <span style="color:#8B008B"> '''Caution:''' Very toxic and light sensitive (store solution in a dark glass vial).</span>. | ||
Revision as of 13:47, 18 September 2018
- high-resolution terminology - matching measurements at high-resolution
Rotenone
Description
Rotenone is an inhibitor of Complex I (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also Complex II-linked substrate state, S(Rot).
Abbreviation: Rot
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRFR
- Rot: Rotenone (C23H22O6); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).
- Caution: Very toxic and light sensitive (store solution in a dark glass vial)..
- Preparation of 1 mM stock solution (dissolved in 99.9% ethanol)
- Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).
- Divide into 100 µl aliquots (use dark glass vials).
- Store frozen at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.5 µM.
Inhibition of CI by rotenone
- Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787:384–92. >>PMC2724837
