Rotenone

high-resolution terminology - matching measurements at high-resolution

Rotenone

Description

Rotenone is an inhibitor of Complex I (CI) and thus inhibits NADH oxidation. It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of CI. See also S_(Rot).

Abbreviation: Rot

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MiPNet03.02

MitoPedia topics: Inhibitor 

Application in HRR

Rot: Rotenone (C₂₃H₂₂O₆); Sigma R 8875, 1 g, store commercial stock at RT; FW = 394.42 (≥ 95%).

Caution: Very toxic and light sensitive (store solution in a dark glass vial). Use fresh aliquots for every experimental day!.

Preparation of 1 mM stock solution (dissolved in 99.9% ethanol):

1. Weigh 0.39 mg of rotenone and dissolve in 1 ml of ethanol absolute (99.9%).

2. Divide into 100 µl aliquots (use dark glass vials).

3) Store frozen at -20 °C.

O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
• Final concentration: 0.5 µM.

Inhibition of CI by rotenone

• Fato R, Bergamini C, Bortolus M, Maniero AL, Leoni S, Ohnishi T, Lenaz G (2009) Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim Biophys Acta 1787: 384–392. >>PMC2724837