Difference between revisions of "Respiratory state"

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high-resolution terminology - matching measurements at high-resolution

Respiratory state

Description

Respiratory states of mitochondrial preparations and living cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling control state and the electron transfer-pathway state.

Reference: Gnaiger 2014 MitoPathways

MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, Recommended

MitoPedia methods: Respirometry, Spectrophotometry

MitoPedia topics: EAGLE

Communicated by Gnaiger E 2010-10-21, edited 2016-08-26. Edited by [[]] 2020-04-23.

Coupling control states

Coupling control states and CCR of mitochondrial preparations:

OXPHOS, LEAK, ET-pathway (P, L, E) - corrected for ROX. CCR: L/E, P/E, L/P

Coupling control states of living cells:

ROUTINE, LEAK, ET-pathway (R, L, E) - corrected for ROX. CCR: L/E, R/E, L/R

Electron transfer-pathway state

Electron transfer-pathway state, are defined by substrate type (at saturating concentration):

Living cells: endogenous, exogenous substrate control
Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I (NADH Electron transfer-pathway state), CII (succinate-pathway), or other branches converging at the Q-junction, particularly with fatty acid oxidation (fatty acid oxidation pathway control state), alpha-glycerophosphate, or substrate combinations applied for reconstitution of TCA cycle function (e.g. NS-pathway control state, etc.).

Control by substrate concentration: Kinetic control states:

Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O₂; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as J_max, K_m', c₅₀, or p₅₀.
Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the K_I'.

Classical respiratory states

Chance and Williams (1955):

State 1, State 2, State 3, State 4, State 5. CCR: RCR

Derived respiratory states:

State 3u, State 4o. CCR: UCR

Thermodynamics of irreversible processes:

Static head, Level flow

MitoPedia: Respiratory states

» See the complete MitoPedia: Respiratory states

» MitoPedia: SUIT

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-  Communicated by [[Gnaiger E]] 2010-10-21, edited 2016-08-26.
+  Communicated by [[Gnaiger E]] 2010-10-21, edited 2016-08-26. Edited by [[]] 2020-04-23.
 [[Image:P.jpg|right|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|right|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|right|link=ET-capacity|ETS]] [[Image:L.jpg|right|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|right|link=Residual oxygen consumption|ROX]]
 == Coupling control states ==
-::: Coupling states and ''[[CCR]]'' of mitochondrial preparations:
+::: Coupling control states and ''[[CCR]]'' of mitochondrial preparations:
-::::* [[OXPHOS]], [[LEAK]], [[ETS]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]''
+::::* [[OXPHOS]], [[LEAK]], [[Electron transfer-pathway|ET-pathway]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]''
-::: Coupling states of living cells:
+::: Coupling control states of living cells:
-::::* [[ROUTINE]], [[LEAK]], [[ETS]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]''
+::::* [[ROUTINE]], [[LEAK]], [[Electron transfer-pathway|ET-pathway]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]''
 == Electron transfer-pathway state ==
-:::: Electron transfer-pathway state, ''[[PCS]]'', are defined by substrate type (at saturating concentration):
+:::: Electron transfer-pathway state, are defined by substrate type (at saturating concentration):
-::::* Living cells: [[endogenous]], [[exogenous]] substrate control
+::::* Living cells: endogenous, exogenous substrate control
-::::* Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I, CII, or other branches converging at the Q-junction, particularly with [[fatty acid oxidation]], alpha-[[glycerophosphate]], ([[Complex I-linked substrate state]], [[Complex II-linked substrate state]], etc.), or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[Complex I&II-linked substrate state]], etc.).
+::::* Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I ([[NADH Electron transfer-pathway state]]), CII ([[Succinate-pathway|succinate-pathway]]), or other branches converging at the [[Q-junction]], particularly with [[fatty acid oxidation]] ([[Fatty acid oxidation pathway control state|fatty acid oxidation pathway control state]]), alpha-[[glycerophosphate]], or substrate combinations applied for reconstitution of [[TCA cycle]] function (e.g. [[NS-pathway control state]], etc.).
 ::: Control by substrate concentration: '''Kinetic control states''':