Difference between revisions of "Rasmussen 2001 Am J Physiol Endocrinol Metab"
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|mipnetlab=DK Copenhagen Quistorff B | |mipnetlab=DK Copenhagen Quistorff B | ||
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== MitoEAGLE ''V''<sub>O<sub>2</sub>max</sub>/BME data base == | |||
== MitoEAGLE ''V''<sub> | |||
:::* Human vastus lateralis | :::* Human vastus lateralis | ||
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:::* 24 years | :::* 24 years | ||
:::* Habitually physically active, no training for competition | :::* Habitually physically active, no training for competition | ||
:::* '' | :::* ''H'' = 1.8 m | ||
:::* '' | :::* ''M'' = 75 kg | ||
:::* [[BME]] = 1.10 | :::* [[BME]] = 1.10 | ||
:::* BMI = 23.1 kg·m<sup>-2</sup> | :::* BMI = 23.1 kg·m<sup>-2</sup> | ||
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::::* See also [[Rasmussen 2000 Mol Cell Biochem]], [[Rasmussen 2001 Pflugers Arch]], [[Rasmussen 2004 Comp Biochem Physiol]] | ::::* See also [[Rasmussen 2000 Mol Cell Biochem]], [[Rasmussen 2001 Pflugers Arch]], [[Rasmussen 2004 Comp Biochem Physiol]] | ||
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{{Labeling | |||
|area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | |||
|organism=Human | |||
|tissues=Skeletal muscle | |||
|preparations=Isolated mitochondria | |||
|topics=Flux control | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=F, N, S, Gp, NS | |||
|additional=MitoEAGLE BME, VO2max, BME | |||
}} | |||
Revision as of 03:47, 8 February 2020
| Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Aerobic metabolism of human quadriceps muscle: in vivo data parallel measurements on isolated mitochondria. Am J Physiol Endocrinol Metab 280:E301-7. |
Rasmussen UF, Rasmussen HN, Krustrup P, Quistorff B, Saltin B, Bangsbo J (2001) Am J Physiol Endocrinol Metab
Abstract: The aim of the present study was to examine whether parameters of isolated mitochondria could account for the in vivo maximum oxygen uptake (VO2max) of human skeletal muscle. VO2max and work performance of the quadriceps muscle of six volunteers were measured in the knee extensor model (range 10-18 mmol O2 x min-1 x kg-1 at work rates of 22-32 W/kg). Mitochondria were isolated from the same muscle at rest. Strong correlations were obtained between VO2max and a number of mitochondrial parameters (mitochondrial protein, cytochrome aa3, citrate synthase, and respiratory activities). The activities of citrate synthase, succinate dehydrogenase, and pyruvate dehydrogenase, measured in isolated mitochondria, corresponded to, respectively, 15, 3, and 1.1 times the rates calculated from VO2max. The respiratory chain activity also appeared sufficient. Fully coupled in vitro respiration, which is limited by the rate of ATP synthesis, could account for, at most, 60% of the VO2max. This might be due to systematic errors or to loose coupling of the mitochondrial respiration under intense exercise.
• O2k-Network Lab: DK Copenhagen Quistorff B
MitoEAGLE VO2max/BME data base
- Human vastus lateralis
- 18 males
- 24 years
- Habitually physically active, no training for competition
- H = 1.8 m
- M = 75 kg
- BME = 1.10
- BMI = 23.1 kg·m-2
- VO2max/BM = 54.2 mL·min-1·kg-1
- Isolated mitochondria; 25 °C; GSP; conversions: Gnaiger 2009 Int J Biochem Cell Biol
- JO2,P(NS) = 123.4 µmol·s-1·kg-1 wet muscle mass (37 °C)
- 10.5 µM mt-protein/mg mw
- See also Rasmussen 2000 Mol Cell Biochem, Rasmussen 2001 Pflugers Arch, Rasmussen 2004 Comp Biochem Physiol
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Regulation: Flux control Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, Gp, NS
MitoEAGLE BME, VO2max, BME
