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Difference between revisions of "Pyruvate"

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|description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]]
|description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]]
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' transporter known as the [[pyruvate carrier]].
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' transporter known as the [[pyruvate carrier]].
In the mt-matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]].
In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]].
Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by cyanide. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].
Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by cyanide. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]]
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]]

Revision as of 08:32, 15 April 2014


high-resolution terminology - matching measurements at high-resolution


Pyruvate

Description

Pyruvic acid

Pyruvic acid, C3H4O3, is an alpha-keto acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of lactate dehydrogenase. Pyruvate enters the mitochondrial matrix via a specific low Km' transporter known as the pyruvate carrier. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.

Abbreviation: P

Reference: Gnaiger 2012 MitoPathways, MiPNet09.12



MitoPedia topics: Substrate and metabolite 

Application in HRR

P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 ยฐC; FW = 110.0


Preparation of 2 M stock solution (dissolved in H2O)

Prepare fresh everyday.
1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
2) Add 0.2 ml H2O.
3) Adjust pH to 7.0.


Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: 5 ยตl using a 25 ยตl syringe (2 ml O2k-chamber).
  • Final concentration: 5 mM.