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Difference between revisions of "Pyruvate"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=P
|abbr=P
|description=Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter [[pyruvate carrier]].
|description=[[File:Pyruvic_acid.jpg|left|100px|Pyruvic acid]]
In the mt matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields acetyl-CoA.
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, M''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate as an anion that enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' transporter known as the [[pyruvate carrier]].
In the mt-matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields [[acetyl-CoA]].
Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by cyanide. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]]
|info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]]
|type=Substrate ETS
|type=Substrate ETS
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'''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
'''P: Pyruvate''' (Pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0


<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>




'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):
'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)
:: Prepare fresh everyday.


::1) weigh 44 mg of Pyruvic acid directyl into 1 ml Eppendorf tube
::1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
::2) add 0.2 ml H<sub>2</sub>O
::2) Add 0.2 ml H<sub>2</sub>O.
::3) adjust pH to 7
::3) Adjust pH to 7.0.




'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
'''Oxygraph-2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]


::* Titration volume: 5 µl using a 25 µl syringe
::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
::* Final conc. in 2 ml O2k-chamber: 5 mM
::* Final concentration: 5 mM.

Revision as of 08:25, 15 April 2014


high-resolution terminology - matching measurements at high-resolution


Pyruvate

Description

Pyruvic acid

Pyruvic acid, C3H4O3, is an alpha-keto acid which occurs under physiological conditions mainly as the anion pyruvate-, M, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate as an anion that enters the mitochondrial matrix via a specific low Km' transporter known as the pyruvate carrier. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.

Abbreviation: P

Reference: Gnaiger 2012 MitoPathways, MiPNet09.12



MitoPedia topics: Substrate and metabolite 

Application in HRR

P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0


Preparation of 2 M stock solution (dissolved in H2O)

Prepare fresh everyday.
1) Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
2) Add 0.2 ml H2O.
3) Adjust pH to 7.0.


Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
  • Final concentration: 5 mM.