Difference between revisions of "Pyruvate"
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'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
::1) weigh 44 mg of | ::1) weigh 44 mg of Pyruvic acid directyl into 1 ml Eppendorf tube | ||
::2) add 0.2 ml H<sub>2</sub>O | ::2) add 0.2 ml H<sub>2</sub>O | ||
::3) adjust pH to 7 | ::3) adjust pH to 7 | ||
Revision as of 15:27, 8 October 2013
- high-resolution terminology - matching measurements at high-resolution
Pyruvate
Description
Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter pyruvate carrier. In the mt matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA.
Abbreviation: P
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
Caution: Prepare fresh everyday!
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 44 mg of Pyruvic acid directyl into 1 ml Eppendorf tube
- 2) add 0.2 ml H2O
- 3) adjust pH to 7
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM
