Difference between revisions of "Pyruvate"
From Bioblast
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|description=Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter [[pyruvate carrier]]. | |description=Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter [[pyruvate carrier]]. | ||
In the mt matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields acetyl-CoA. | In the mt matrix the oxidative decarboxylation of '''pyruvate''' is catalyzed by [[pyruvate dehydrogenase complex]] and yields acetyl-CoA. | ||
|info=[ | |info=[http://www.oroboros.at/?Gnaiger_2012_MitoPathways Gnaiger 2012 MitoPathways], [[MiPNet09.12]] | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | |||
{{MitoPedia methods|type=Substrate ETS | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
Revision as of 12:43, 9 January 2013
- high-resolution terminology - matching measurements at high-resolution
Pyruvate
Description
Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter pyruvate carrier. In the mt matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA.
Abbreviation: P
Reference: Gnaiger 2012 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
Caution: Prepare fresh everyday!
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube
- 2) add 0.2 ml H2O
- 3) adjust pH to 7
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM
