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Difference between revisions of "Pyruvate"

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'''Answer:'''
'''Answer:'''
Pyruvate alone is not sufficient to support NADH-linked respiration. In order to do so you will need to combine at least a 2<sup>nd</sup> NADH-linked substrate (''e.g.,'' Glutamate, Malate...).
Pyruvate alone is not sufficient to support NADH-linked respiration. In order to do so you will need to combine at least a 2<sup>nd</sup> NADH-linked substrate (''e.g.,'' Glutamate, Malate...). ''See'' [[Gnaiger 2014 MitoPathways]]
Β 
Additionally, you may consult some of the publications from ''Drosophila melanogaster'' mitochondria: https://wiki.oroboros.at/index.php/O2k-Publications:_Drosophila
Additionally, you may consult some of the publications from ''Drosophila melanogaster'' mitochondria:[[O2k-Publications:_Drosophila]]

Revision as of 13:38, 20 March 2020


high-resolution terminology - matching measurements at high-resolution


Pyruvate

Description

Pyruvic acid

Pyruvic acid, C3H4O3, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of lactate dehydrogenase. Pyruvate enters the mitochondrial matrix via a specific low Km' H+/monocarboxylate cotransporter known as the pyruvate carrier. Similarly, the plasma membrane of many cell types has H+/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to living cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA. Pyruvate alone cannot support respiration of mt-preparations from most tissues. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.

Abbreviation: P

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12 O2k-Titrations


MitoPedia topics: Substrate and metabolite 

Application in HRR

P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 Β°C; FW = 110.0
Preparation of 2 M stock solution (dissolved in H2O)
  1. Prepare fresh everyday.
  2. Weigh 44 mg of pyruvic acid directyl into a 0.5 mL Eppendorf tube.
  3. Add 0.2 mL H2O.
  4. Adjust pH to 7.0.


O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 5 Β΅L using a 25 Β΅L syringe (2 mL O2k-chamber).
  • Final concentration: 5 mM.

Troubleshooting

Unstable respiration while using Pyruvate as the only substrate

Question:

I am evaluating mitochondrial respiration from Drosophila melanogaster using Pyruvate, ADP, and Cytochrome C. However, I do not achieve a steady state level in OXPHOS.

Any advice would be appreciated. The data is attached (2019-07-17).

Ticket2019072631000015.png

Answer: Pyruvate alone is not sufficient to support NADH-linked respiration. In order to do so you will need to combine at least a 2nd NADH-linked substrate (e.g., Glutamate, Malate...). See Gnaiger 2014 MitoPathways

Additionally, you may consult some of the publications from Drosophila melanogaster mitochondria:O2k-Publications:_Drosophila