Difference between revisions of "Pyruvate"
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Revision as of 16:37, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Pyruvate
Description
Pyruvate as a product of glycolysis is an anion that enters mitochondrial matrix via a specific low Km transporter pyruvate carrier. In the mt matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase complex and yields acetyl-CoA.
Abbreviation: P
Reference: MiPNet11.04, MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
P: Pyruvate (Pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at R.T.: FW = 110.0)
Preparation of 2 M stock solution (dissolved in H2O):
- !!Prepare fresh every day!!
- 1) weigh 44 mg of L-Glutamic acid directyl into 1 ml Eppendorf tube
- 2) add 0.2 ml H2O
- 3) adjust pH to 7
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 5 mM
