Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Pyruvate"

From Bioblast
Line 3: Line 3:
|description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]]
|description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]]
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' H<sup>+</sup>/monocarboxylate cotransporter known as the [[pyruvate carrier]]. Similarly, the plasma membrane of many cell types has H<sup>+</sup>/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to intact cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]]. Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by [[cyanide]]. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' H<sup>+</sup>/monocarboxylate cotransporter known as the [[pyruvate carrier]]. Similarly, the plasma membrane of many cell types has H<sup>+</sup>/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to intact cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]]. Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by [[cyanide]]. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]].
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]]
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12 O2k-Titrations]]
|type=Substrate ETS
}}
{{MitoPedia methods|type=Substrate ETS
}}
}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
|mitopedia topic=Substrate and metabolite
|type=Substrate ETS
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==


'''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
::: '''P: Pyruvate''' (pyruvic acid, sodium salt, C<sub>3</sub>H<sub>3</sub>O<sub>3</sub>Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
 
 


'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)
::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)
: Prepare fresh everyday.
:::: Prepare fresh everyday.


::1. Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
::::# Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
::2. Add 0.2 ml H<sub>2</sub>O.
::::# Add 0.2 ml H<sub>2</sub>O.
::3. Adjust pH to 7.0.
::::# Adjust pH to 7.0.




'''Oxygraph-2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
::: '''Oxygraph-2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]


::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
::::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
::* Final concentration: 5 mM.
::::* Final concentration: 5 mM.

Revision as of 11:51, 27 March 2016


high-resolution terminology - matching measurements at high-resolution


Pyruvate

Description

Pyruvic acid

Pyruvic acid, C3H4O3, is an alpha-keto monocarboxylic acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of lactate dehydrogenase. Pyruvate enters the mitochondrial matrix via a specific low Km' H+/monocarboxylate cotransporter known as the pyruvate carrier. Similarly, the plasma membrane of many cell types has H+/monocarboxylate cotransporter activity and pyruvate can thus be added as a substrate to intact cells. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.

Abbreviation: P

Reference: Gnaiger 2014 MitoPathways, MiPNet09.12 O2k-Titrations


MitoPedia topics: Substrate and metabolite 

Application in HRR

P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 °C; FW = 110.0
Preparation of 2 M stock solution (dissolved in H2O)
Prepare fresh everyday.
  1. Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
  2. Add 0.2 ml H2O.
  3. Adjust pH to 7.0.


Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
  • Final concentration: 5 mM.