Difference between revisions of "Puchowicz 2004 Mitochondrion"
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{{Publication | {{Publication | ||
|title=Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria-case studies. Mitochondrion 4: 377- | |title=Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria-case studies. Mitochondrion 4:377-85. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16120399 PMID:16120399] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/16120399 PMID:16120399] | ||
|authors=Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL | |authors=Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL | ||
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|organism=Human | |organism=Human | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Inner mt-membrane transporter, TCA cycle and matrix dehydrogenases | |||
|topics=Substrate; Glucose; TCA Cycle | |||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
}} | }} | ||
Revision as of 12:50, 13 February 2015
| Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Oxidative phosphorylation analysis: assessing the integrated functional activity of human skeletal muscle mitochondria-case studies. Mitochondrion 4:377-85. |
Puchowicz MA, Varnes ME, Cohen BH, Friedman NR, Kerr DS, Hoppel CL (2004) Mitochondrion
Abstract: Oxidative phosphorylation analysis, performed on freshly-isolated mitochondria, assesses the integrated function of the electron transport chain (ETC) coupled to ATP synthesis, membrane transport, dehydrogenase activities, and the structural integrity of the mitochondria. In this review, a case study approach is employed to highlight detection of defects in the adenine nucleotide translocator, the pyruvate dehydrogenase complex, fumarase, coenzyme Q function, fatty acid metabolism, and mitochondrial membrane integrity. Our approach uses the substrates glutamate, pyruvate, 2-ketoglutarate (coupled with malonate), malate, and fatty acid substrates (palmitoylcarnitine, octanoylcarnitine, palmitoyl-CoA (with carnitine), octanoyl-CoA (with carnitine), octanoate and acetylcarnitine) in addition to succinate, durohydroquinone and TMPD/ascorbate to uncover metabolic defects that would not be apparent from ETC assays performed on detergent-solubilized mitochondria. • Keywords: Oxidative phosphorylation; Skeletal muscle mitochondria; Adenine nucleotide translocator; Pyruvate dehydrogenase complex; Uncoupling; Carnitine palmitoyltransferase
• O2k-Network Lab: US_OH Cleveland_Hoppel CL
Labels:
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Enzyme: Inner mt-membrane transporter, TCA cycle and matrix dehydrogenases
Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Coupling state: OXPHOS
