Pichaud 2013 Abstract MiP2013

Pichaud N, Blier PU(2013) Importance of mitochondrial haplotypes in the expression of metabolic phenotypes under different conditions. Mitochondr Physiol Network 18.08.

Link:

Pichaud N, Blier PU (2013)

Event: MiP2013

Differential expression of genes mediated by environmental parameters has the potential to influence the organismal phenotype. Considering the central importance of mitochondria in physiological processes such as senescence and life history traits, we hypothesize that expression of mitochondrial genes, under different environmental conditions, should be under strong evolutionary constraints. Integrity of mitochondrial functions requires a synergistic interaction between the mitochondrial and nuclear genomes with proteins produced from mtDNA genes interacting with proteins imported from nuclear encoded genes to produce a functional mitochondrial electron transfer system (ETS). Using permeabilized fibers of Drosophila expressing different mtDNA haplotypes in a homogenous nuclear background, we investigated the effect of several conditions such as aging, temperature and diet on mitochondrial functions. We showed that different set of conditions may trigger the expression of a particular phenotype caused by mtDNA divergences. This is of paramount importance to understand the influence of the environment on mitochondrial evolution in a wide variety of species including humans and has the potential to provide a cohesive picture of the underlying mechanisms of co-evolved ETS Complexes.

• O2k-Network Lab: CA Rimouski Blier PU

Labels: MiParea: mt-Biogenesis;mt-density, mtDNA;mt-genetics  Pathology: Aging; senescence"Aging; senescence" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property.  Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.  Organism: Drosophila  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue  Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., Marker Enzyme"Marker Enzyme" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property., TCA Cycle and Matrix Dehydrogenases"TCA Cycle and Matrix Dehydrogenases" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.  Regulation: ADP, Flux control, Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Pi"Pi" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Uncoupler  Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, Spectrophotometry"Spectrophotometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Affiliations and author contributions

Laboratoire de Biologie Intégrative, Université du Québec à Rimouski, Canada.

Email: [email protected]