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Pham 2014 Am J Physiol

From Bioblast
Publications in the MiPMap
Pham T, Loiselle D, Power A, Hickey AJ (2014) Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am J Physiol 307:C499โ€“507.

ยป PMID: 24920675

Pham T, Loiselle D, Power A, Hickey AJ (2014) Am J Physiol

Abstract: As approximately 80% of diabetics die from heart failure, understanding diabetic cardiomyopathy is crucial. Mitochondria occupy 35-40% of mammalian cardiomyocyte volume, supply 95% of the hearts' ATP, and diabetic heart mitochondria show impaired structure, arrangement and function. We predict that bioenergetic efficiencies are present in diabetic heart mitochondria; therefore we explored mitochondrial proton and electron handling by linking oxygen flux within streptozotocin (STZ)-induced-diabetic Sprague-Dawley rat heart tissues, to steady-state ATP synthesis, Reactive Oxygen Species (ROS) production, and mitochondrial membrane potential (ฮ”ฮจ). By coupling high-resolution respirometers with purpose-built fluorometers, we followed Magnesium Green (ATP synthesis), Amplex Ultra Red (ROS production), and safranin-O (ฮ”ฮจ). Relative to control rats, the mass-specific respiration of STZ-diabetic hearts was depressed in oxidative phosphorylating (OXPHOS) states. Steady-state ATP synthesis capacity was almost a third lower in STZ-diabetic heart and relative to O2 flux, this equates to an estimated 12% depression in OXPHOS efficiency. However, with anoxic transition, STZ-diabetic and control heart tissues showed similar ATP hydrolysis capacities through reversal of the F1/F0 ATP-synthase. STZ-diabetic cardiac mitochondria also produced more net ROS relative to oxygen flux (ROS/O) in OXP. While ฮ”ฮจ did not differ between groups, the time to develop ฮ”ฮจ with the onset of OXPHOS was protracted in STZ-diabetic mitochondria. ROS/O is higher in life-like OXPHOS states and potential delays in the time to develop ฮ”ฮจ may delay ATP synthesis with inter-beat fluctuations in ADP concentrations. Whereas diabetic cardiac mitochondria produce less ATP in normoxia, they consume as much ATP in anoxic infarct-like states. โ€ข Keywords: Diabetic cardiomypathy, Anoxia, Efficiency, Mitochondria, Oxidative phosphorylation, Safranin, Amplex Red, Magnesium green

โ€ข O2k-Network Lab: NZ Auckland Hickey AJ


Labels: MiParea: Respiration, mt-Medicine  Pathology: Diabetes, Myopathy  Stress:Hypoxia, RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., Mitochondrial disease  Organism: Rat  Tissue;cell: Heart  Preparation: Homogenate 

Regulation: Coupling efficiency;uncoupling, mt-Membrane potential  Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, O2k-Fluorometer 


Demonstration of using Amplex Red, Safranin and Magnesium green methods with the O2k-Fluoresececen Module.