Park 2007 Sensors IEEE
| Park J, Chang J, Choi M, Pak JJ, DY Lee, Pak YK (2007) Sensors microfabirated Clark-type sensor for measuring dissolved oxygen. Meter (Strathkelvin Instruments) and the Oroboros Oxygraph-2k (Oroboros Instruments) for measuring the oxygen respiration level of cells are currently widely. |
» IEEE
Park J, Chang J, Choi M, Pak JJ, DY Lee, Pak YK (2007) IEEE
Abstract: This paper presents a microfabricated Clark-type sensor which exactly can measure dissolved oxygen in the cell containing solution. We designed and fabricated a microfabricated Clark-type sensor for measuring the oxygen respiration level of few cells. The microfabricated Clark-type sensor is composed of 3-electrodes on a glass substrate, a FEP (Fluorinated ethylene propylene) oxygen-permeable membrane, and PDMS (Polydimethylsiloxane) reservoir for storing few cells containing solution. The microfabricated Clark-type sensor was fabricated using MEMS technology. Thin-film Ag/AgCl was employed as a reference electrode and its durability was verified by obtaining a stable open circuit potential versus a commercial Ag/AgCl electrode for 2 hours. Response time, reproducibility and linearity of the fabricated oxygen sensor were examined by amperometry. The fabricated Clark-type sensor showed 40 sec of 90% response time, reproducibility with 1.37 nA standard deviation of 97.03 nA mean in the full-oxygen state and 0.26 nA standard deviation of 3.25 nA mean in the zero-oxygen state, and an excellent linearity with a correlation coefficient of 0.994.
Labels:
Organism: Other Mammal"Other Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
