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Difference between revisions of "PGM-pathway control state"

From Bioblast
m (Cardoso Luiza moved page PGM to PGM-pathway control state: To match nomenclature of other pages: PM-pathway control state, GM-pathway control state)
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|description=[[File:PGM.jpg|left|200px|PGM]] '''PGM''': [[Pyruvate]] & [[Glutamate]] & [[Malate]].
|description=[[File:PGM.jpg|left|200px|PGM]] '''PGM''': [[Pyruvate]] & [[Glutamate]] & [[Malate]].


'''MitoPathway control state:''' [[ N| NADH Electron transfer-pathway state]]
'''MitoPathway control state:''' [[ N| NADH electron transfer-pathway state]]


'''SUIT protocol:''' [[SUIT-001]], [[SUIT-012]], [[SUIT-014]]
'''SUIT protocol:''' [[SUIT-001]], [[SUIT-012]], [[SUIT-014]]
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}}
}}
::: ''More details''  
::: ''More details''  
::::» [[NADH Electron transfer-pathway state]]
::::» [[NADH electron transfer-pathway state]]
::::» [[Additive effect of convergent electron flow]]
::::» [[Additive effect of convergent electron flow]]
::::» [[Respiratory_complexes#Respiratory_complexes_-_more_than_five |Respiratory complexes - more than five]]
::::» [[Respiratory_complexes#Respiratory_complexes_-_more_than_five |Respiratory complexes - more than five]]

Revision as of 13:36, 26 August 2020


high-resolution terminology - matching measurements at high-resolution


PGM-pathway control state

Description

PGM

PGM: Pyruvate & Glutamate & Malate.

MitoPathway control state: NADH electron transfer-pathway state

SUIT protocol: SUIT-001, SUIT-012, SUIT-014

Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO2, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the glutamate-aspartate carrier and reacts with oxaloacetate in the transaminase reaction to form aspartate and oxoglutarate. Glutamate as the sole substrate is transported by the electroneutral glutamate-/OH- exchanger, and is oxidized in the mitochondrial matrix by glutamate dehydrogenase to α-ketoglutarate ( 2-oxoglutarate), representing the glutamate-anaplerotic pathway control state. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.

Abbreviation: PGM

Reference: Gnaiger 2014 MitoPathways - Chapter 5.3


MitoPedia concepts: SUIT state 

More details
» NADH electron transfer-pathway state
» Additive effect of convergent electron flow
» Respiratory complexes - more than five
» Convergent electron flow

PGML

PGM pathway in the LEAK state can be evaluated in the following SUIT protocols:

PGMP

PGM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

PGME

PGM pathway in the ET state can be evaluated in the following SUIT protocols:


Discussion