Difference between revisions of "Oroboros Laboratory"
From Bioblast
| Line 7: | Line 7: | ||
:::: The '''Oroboros MitoFit Laboratory''' (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for | :::: The '''Oroboros MitoFit Laboratory''' (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for High-Resolution FluoRespirometry (HRFR). The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], June 2013. It comprises 140 sqm which are divided into the office and research areas. | ||
=== O2k instruments === | === O2k instruments === | ||
| Line 13: | Line 13: | ||
:::: '''28 O2k-Chambers (14 O2k)''' | :::: '''28 O2k-Chambers (14 O2k)''' | ||
::::* | ::::* Fourteen O2k instruments for HRFR (28 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations. | ||
::::* Two additional O2ks (4 chambers) are devoted to instrumental development. | ::::* Two additional O2ks (4 chambers) are devoted to instrumental development. | ||
| Line 21: | Line 21: | ||
=== | === The MitoFit Centre of Excellence === | ||
:::: | ::::* Two labs, one team: Oroboros- and DSL-MitoFit Laboratories. | ||
:::: | :::: The '''DSL-MitoFit Lab''' at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particulary devoted to mitochondrial reseearch on cells isolated from blood samples and cultured cells. | ||
:::: | :::: '''Oroboros Instruments''' collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions. | ||
:::: According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world. | |||
=== | [[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] | ||
=== OXPHOS analysis === | |||
:::: | :::: SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using | ||
:::: | ::::* coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions; | ||
::::* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]]). | |||
::::» [[Oroboros_contact#Current_projects |'''Current projects and collaborators''']] | ::::» [[Oroboros_contact#Current_projects |'''Current projects and collaborators''']] | ||
Revision as of 14:45, 2 August 2017
Oroboros Laboratory
- Two labs - one team
- The Oroboros MitoFit Laboratory (MitoFit Lab, 'Mitochondriales Forschungslabor') is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for High-Resolution FluoRespirometry (HRFR). The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, June 2013. It comprises 140 sqm which are divided into the office and research areas.
O2k instruments
- 28 O2k-Chambers (14 O2k)
- Fourteen O2k instruments for HRFR (28 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules or the NextGen-O2k configuration are available for research and biomedical applications. The newest instrumental designs are used for rigorous testing of our innovations.
- Two additional O2ks (4 chambers) are devoted to instrumental development.
- » More details: Oroboros Support
The MitoFit Centre of Excellence
- Two labs, one team: Oroboros- and DSL-MitoFit Laboratories.
- The DSL-MitoFit Lab at the Daniel Swarovski Research Laboratory (DSL), Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck is equipped by Oroboros Instruments with eight O2k-FluoRespirometers (16 chambers), particulary devoted to mitochondrial reseearch on cells isolated from blood samples and cultured cells.
- Oroboros Instruments collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous international research institutions.
- According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
OXPHOS analysis
- SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
- coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions;
- various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry).
» Picked up - reading and discussion
