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Difference between revisions of "OroboPOS-Connector MitoPedia"

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== Functions ==
== Functions ==

Revision as of 08:20, 27 May 2020


high-resolution terminology - matching measurements at high-resolution


OroboPOS-Connector MitoPedia

Description

OroboPOS-Connector (blue POM), with male connection to OroboPOS sensor head (POS) and with cable and male plug fitting into O2k-Main Unit.Two units of this item are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer), mounted on the OroboPOS-Holders at the O2k-Main Unit.


Reference: OroboPOS-Connector





MitoPedia O2k and high-resolution respirometry: O2k hardware 






Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 




Functions

The OroboPOS-Connector has several different functions:
  • It mechanically presses the POS against the glass chamber, ensuring a tight fit.
  • It connects the POS electronically to the Main unit transmitting the signal.
  • In O2k Series A-C it also performs the current to voltage conversion / amplification. For Series D and upwards this function has been incorporated into the Main unit. For this reason OroboPOS connectors for Series A-C and for Series D are NOT interchangeable.
The OroboPOS-Connector is sensitive to damage by ESD when disconnected from the O2k-Main Unit and when the POS head is removed. Further details: MiPNet14.01_ESD-damage.

Cleaning

When experimental media or electrolyte (during service of the polarographic oxygen sensor OroboPOS leak into the mechanical part of the OroboPOS-Connector, operation of the spring mechanism may be restricted. In such cases, the body of the OroboPOS-Connector should be washed with distilled water. To do so, screw the OroboPOS head onto the OroboPOS-Connector. Make sure that the plug connecting to the O2k-Main Unit does not get wet (protect with parafilm). Rinse the body of the OroboPOS-Connector with distilled water, pulling the spring-loaded part at the base several times. Salt crystals and media clogging the mechanical part are thus washed out. Wipe off the water while again pulling down the spring-loaded part.

Cleaning the electrical connections

  1. Unscrew the OroboPOS and check the electrical connections (the gold pin and thread on the OroboPOS and OroboPOS-Connector).
  2. Remove any contaminating grease and moisture with a fine paper cloth.
  3. Soak a paper cloth in ethanol and carefully clean the gold pin and threads. If the gold pin and thread on the OroboPOS-Connector are discolored or appear stained, clean repeatedly with a paper towel soaked in water and subsequently in ethanol, until no more traces appear on the paper towel used for cleaning.
  • If the O2k is used in a humid environment, the connector can be dried in an oven at 50Β°C for 1-2h. Application of a drop of contact oil (as previously recommended) is generally not required, but when done make sure only very little oil is applied to each of the golden pins and threads on POS connector and POS. Follow the procedure with an Oxygen sensor test as a quality control of proper OroboPOS-Connector and sensor performance.

Replacing the OroboPOS-Connector

If you need to replace the Orobo-POS Connector, an OroboPOS-Connector Service may be possible.