Ojuka 2016 Am J Physiol Endocrinol Metab
| Ojuka E, Andrew B, Bezuidenhout N, George S, Maarman G, Madlala HP, Mendham A, Osiki PO (2016) Measurement of Ξ²-oxidation capacity of biological samples by respirometry: a review of principles and substrates. Am J Physiol Endocrinol Metab 310:E715-23. |
Ojuka E, Andrew B, Bezuidenhout N, George S, Maarman G, Madlala HP, Mendham A, Osiki PO (2016) Am J Physiol Endocrinol Metab
Abstract: Oxidation of fatty acids is a major source of energy in the heart, liver, and skeletal muscle. It can be measured accurately using respirometry in isolated mitochondria, intact cells, and permeabilized cells or tissues. This technique directly measures the rate of oxygen consumption or flux at various respiratory states when appropriate substrates, uncouplers, and inhibitors are used. Acylcarnitines such as palmitoylcarnitine or octanoylcarnitine are the commonly used substrates. The Ξ²-oxidation pathway is prone to feedforward inhibition resulting from accumulation of short-chain acyl-CoA and depletion of CoA, but inclusion of malate or carnitine prevents accumulation of these intermediaries and CoA depletion. β’ Keywords: Beta-oxidation, Palmitoylcarnitine, Octanylcarnitine, Malate, Carnitine, Substrate combinations, Respirometry
β’ O2k-Network Lab: ZA Cape Town Ojuka EO
Labels: MiParea: Respiration, Instruments;methods
Organism: Human, Mouse, Rat
Tissue;cell: Skeletal muscle, Liver
Preparation: Permeabilized tissue, Isolated mitochondria
Regulation: Fatty acid Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. Pathway: F HRR: Oxygraph-2k
Review, 2016-07
