OctM pathway control state

high-resolution terminology - matching measurements at high-resolution

OctM pathway control state

Description

OctM: Octanoylcarnitine & Malate.

MitoPathway control state: F

SUIT protocols: SUIT-002, SUIT-015, SUIT-016, SUIT-017

Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration. Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. High concentration of malate, typically 2 mM, saturates the N-pathway.

Abbreviation: OctM

Reference: MiPNet21.06 SUIT RP, SUIT reference protocol

More details

» Fatty acid oxidation pathway control state

OctM_L

FAO in the LEAK state can be evaluated in the following SUIT protocols:

• SUIT-005

• DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011

• SUIT-015
• SUIT-016
• SUIT-017

OctM_P

FAO in the OXPHOS state can be evaluated in the following SUIT protocols:

• SUIT-002

• DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
• DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
• DL-Protocol for permeabilized cells (pce): SUIT-002 O2 ce-pce D007

• SUIT-005

• DL-Protocol for permeabilized fibers (pfi): SUIT-005 O2 pfi D011

• SUIT-015
• SUIT-016
• SUIT-017

OctM_E

FAO in the ET state can be evaluated in the following SUIT protocols:

---

MitoPedia concepts: SUIT state