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Difference between revisions of "O2k-Specifications"

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{{Product
{{Technical support}}
|description='''O2k versus multiwell respirometer''': '''O2k''' stands for Oroboros O2k and '''High-Resolution FluoRespirometry''', meeting powerful quality criteria securing '''high output''' and pioneering state-of-the-art [[Gnaiger_2012_MitoPathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. ''If youā€™re using a biased instrument, it doesnā€™t matter how many measurements you take ā€“ youā€™re aiming at the wrong target'' ([[Silver 2012 Penguin Press]]).
__TOC__
|product id=* [[Media:MiPNet18.10 O2k specifications.pdf|'''Open Access: O2k specifications.pdf''']]
{{MitoPedia without banner
|product type=Catalogue, Feedback
|abbr=
|info=[[MiPNet18.10 O2k specifications|Bioblast pdf]], [http://wiki.oroboros.at/index.php/File:Flyer_backside_.png Flyer backside]
|description=[[Image:O2k-Info.png|right|30px|link=Oroboros O2k]]'''O2k versus multiwell respirometer''': '''O2k''' stands for Oroboros O2k and '''[[high-resolution respirometry]]''', meeting powerful quality criteria securing '''high output''' and pioneering state-of-the-art [[Gnaiger_2012_MitoPathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. ''If youā€™re using a biased instrument, it doesnā€™t matter how many measurements you take ā€“ youā€™re aiming at the wrong target'' ([[Silver 2012 Penguin Press]]).
|product image=[[Image:O2k-Info.png|right|30px|link=Oroboros O2k]]
|info= [[MiPNet18.10 O2k-Specifications]], [http://wiki.oroboros.at/images/9/93/Flyer_backside_.png Flyer with specification summary]
}}
}}
{{MitoPedia O2k and high-resolution respirometry
{{MitoPedia O2k and high-resolution respirometry
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<br />
<br />


{{Publication
|title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=OROBOROS INSTRUMENTS]] O2k-FluoRespirometer: specifications for respirometry and comprehensive OXPHOS analysis.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/d/db/MiPNet18.10_O2k_specifications.pdf|Bioblast pdf]] Ā» [http://www.bioblast.at/index.php/File:MiPNet18.10_O2k_specifications.pdf Versions]
|authors=Oroboros
|year=2017-08-08
|journal=MiPNet
|abstract='''Gnaiger E (2017) O2k-FluoRespirometer: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(07): 1-8.''' Ā» [http://www.bioblast.at/index.php/File:MiPNet18.10_O2 specifications.pdf Versions]
|keywords=O2k-Specifications
|mipnetlab=AT Innsbruck Oroboros
}}
{{Labeling
|area=Respiration, Instruments;methods
|organism=Human
|tissues=Fibroblast
|preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP
|instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory
}}




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== Selected references ==
== Selected references ==


Ā  [[Talk:MiPNet18.10 O2k versus multiwell | Discussion]]
Ā  [[Comparison of respirometric methods]] - [[Talk:Comparison of respirometric methods | Discussion]]


::::* Horscroft JA, Kotwica AO, Laner V, West JA, Hennis PJ, Levett DZH, Howard DJ, Fernandez BO, Burgess SL, Ament Z, Gilbert-Kawai ET, Vercueil A, Landis BD, Mitchell K, Mythen MG, Branco C, Johnson RS, Feelisch M, Montgomery HE, Griffin JL, Grocott MPW, Gnaiger E, Martin DS, Murray AJ (2017) Metabolic basis to Sherpa altitude adaptation. Proc Natl Acad Sci U S A 114:6382ā€“7. - [[Horscroft 2017 Proc Natl Acad Sci U S A |Ā»Bioblast linkĀ«]]
::::* Horscroft JA, Kotwica AO, Laner V, West JA, Hennis PJ, Levett DZH, Howard DJ, Fernandez BO, Burgess SL, Ament Z, Gilbert-Kawai ET, Vercueil A, Landis BD, Mitchell K, Mythen MG, Branco C, Johnson RS, Feelisch M, Montgomery HE, Griffin JL, Grocott MPW, Gnaiger E, Martin DS, Murray AJ (2017) Metabolic basis to Sherpa altitude adaptation. Proc Natl Acad Sci U S A 114:6382ā€“7. - [[Horscroft 2017 Proc Natl Acad Sci U S A |Ā»Bioblast linkĀ«]]
Line 66: Line 46:
::::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - [[Makrecka-Kuka 2015 Biomolecules |Ā»Bioblast linkĀ«]]
::::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - [[Makrecka-Kuka 2015 Biomolecules |Ā»Bioblast linkĀ«]]


::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. Ā»[[Gnaiger 2014 MitoPathways |Bioblast pdfĀ«]]
::::* Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun:112 pp. Ā»[[Gnaiger 2020 BEC MitoPathways |Bioblast linkĀ«]]


::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |Ā»Bioblast pdfĀ«]] Ā 
::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |Ā»Bioblast linkĀ«]] Ā 


::::* Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. [[Votion 2012 PLoS One ||Ā»Open AccessĀ«]]
::::* Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. [[Votion 2012 PLoS One ||Ā»Bioblast linkĀ«]]


::::* Lemieux H, Semsroth S, Antretter H, Hƶfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729ā€“38. - [[Lemieux 2011 Int J Biochem Cell Biol |Ā»Bioblast linkĀ«]]
::::* Lemieux H, Semsroth S, Antretter H, Hƶfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729ā€“38. - [[Lemieux 2011 Int J Biochem Cell Biol |Ā»Bioblast linkĀ«]]
Line 76: Line 56:
::::* Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078ā€“87. - [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol |Ā»Bioblast linkĀ«]]
::::* Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078ā€“87. - [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol |Ā»Bioblast linkĀ«]]


::::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. [[Scandurra 2010 Adv Exp Med Biol |Ā»Bioblast pdfĀ«]]
::::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. - [[Scandurra 2010 Adv Exp Med Biol |Ā»Bioblast linkĀ«]]


::::* Aguirre E, RodrĆ­guez-JuĆ”rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. [[Aguirre 2010 Biochim Biophys Acta ||Ā»Bioblast pdfĀ«]]
::::* Aguirre E, RodrĆ­guez-JuĆ”rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. - [[Aguirre 2010 Biochim Biophys Acta ||Ā»Bioblast linkĀ«]]


::::* AragonĆ©s J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. [[Aragones 2008 Nat Genet |Ā»Open AccessĀ«]]
::::* AragonĆ©s J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. - [[Aragones 2008 Nat Genet |Ā»Bioblast linkĀ«]]


::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |Ā»Bioblast pdfĀ«]]
::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - [[Gnaiger_2008_POS |Ā»Bioblast linkĀ«]]


::::* HĆ¼tter E, Renner K, Pfister G, Stƶckl P, Jansen-DĆ¼rr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. [[Huetter_2004_Biochem J |Ā»Open AccessĀ«]]
::::* HĆ¼tter E, Renner K, Pfister G, Stƶckl P, Jansen-DĆ¼rr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - [[Huetter_2004_Biochem J |Ā»Bioblast linkĀ«]]


::::* Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633ā€“41. [[Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol |Ā»Open AccessĀ«]]
::::* Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633ā€“41. - [[Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol |Ā»Bioblast linkĀ«]]


::::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. [[Gnaiger 2001 Respir Physiol |Ā»Bioblast pdfĀ«]]
::::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger 2001 Respir Physiol |Ā»Bioblast linkĀ«]]


::::* Gnaiger E, MĆ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. [[Gnaiger 2000 Proc Natl Acad Sci U S A |Ā»Open AccessĀ«]]
::::* Gnaiger E, MĆ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. [[Gnaiger 2000 Proc Natl Acad Sci U S A |Ā»Open AccessĀ«]]


::::* Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. [[Steinlechner-Maran_1996_Am J Physiol Cell Physiol |Ā»Bioblast pdfĀ«]]
::::* Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. - [[Steinlechner-Maran_1996_Am J Physiol Cell Physiol |Ā»Bioblast linkĀ«]]
Ā 
::::* Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. [[Gnaiger 1995 J Bioenerg Biomembr |Ā»Bioblast pdfĀ«]]


::::* Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - [[Gnaiger 1995 J Bioenerg Biomembr |Ā»Bioblast linkĀ«]]








{{MitoPedia O2k and high-resolution respirometry
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
}}
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Latest revision as of 09:37, 20 April 2021


                  


O2k-Open Support

O2k-Specifications



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

O2k-Info.png

O2k versus multiwell respirometer: O2k stands for Oroboros O2k and high-resolution respirometry, meeting powerful quality criteria securing high output and pioneering state-of-the-art comprehensive OXPHOS analysis of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. If youā€™re using a biased instrument, it doesnā€™t matter how many measurements you take ā€“ youā€™re aiming at the wrong target (Silver 2012 Penguin Press).


Reference: MiPNet18.10 O2k-Specifications, Flyer with specification summary


MitoPedia O2k and high-resolution respirometry: O2k hardware 


Questions.jpg




Combination of interests

Erich Gnaiger is founder and CEO of Oroboros Instruments, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.


Selected references

Comparison of respirometric methods -  Discussion
  • Horscroft JA, Kotwica AO, Laner V, West JA, Hennis PJ, Levett DZH, Howard DJ, Fernandez BO, Burgess SL, Ament Z, Gilbert-Kawai ET, Vercueil A, Landis BD, Mitchell K, Mythen MG, Branco C, Johnson RS, Feelisch M, Montgomery HE, Griffin JL, Grocott MPW, Gnaiger E, Martin DS, Murray AJ (2017) Metabolic basis to Sherpa altitude adaptation. Proc Natl Acad Sci U S A 114:6382ā€“7. - Ā»Bioblast linkĀ«
  • Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. - Ā»Bioblast linkĀ«
  • Lemieux H, Subarsky P, Doblander C, Wurm M, Troppmair J, Gnaiger E (2017) Impairment of mitochondrial respiratory function as an early biomarker of apoptosis induced by growth factor removal. bioRxiv doi: https://doi.org/10.1101/151480. - Ā»Bioblast linkĀ«
  • Gnaiger E, Boushel R, SĆøndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DĆ­ez-SĆ”nchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. Scand J Med Sci Sports 25 (Suppl 4):126ā€“34. - Ā»Bioblast linkĀ«
  • Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. - Ā»Bioblast linkĀ«
  • Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - Ā»Bioblast linkĀ«
  • Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun:112 pp. Ā»Bioblast linkĀ«
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. Ā»Bioblast linkĀ«
  • Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. |Ā»Bioblast linkĀ«
  • Lemieux H, Semsroth S, Antretter H, Hƶfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729ā€“38. - Ā»Bioblast linkĀ«
  • Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078ā€“87. - Ā»Bioblast linkĀ«
  • Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. - Ā»Bioblast linkĀ«
  • Aguirre E, RodrĆ­guez-JuĆ”rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. - |Ā»Bioblast linkĀ«
  • AragonĆ©s J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. - Ā»Bioblast linkĀ«
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - Ā»Bioblast linkĀ«
  • HĆ¼tter E, Renner K, Pfister G, Stƶckl P, Jansen-DĆ¼rr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - Ā»Bioblast linkĀ«
  • Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633ā€“41. - Ā»Bioblast linkĀ«
  • Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - Ā»Bioblast linkĀ«
  • Gnaiger E, MĆ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. Ā»Open AccessĀ«
  • Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. - Ā»Bioblast linkĀ«
  • Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - Ā»Bioblast linkĀ«



MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry