Difference between revisions of "O2k-Specifications"
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{{Publication | |||
|title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|OROBOROS]] Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. | |||
|info=[[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access-MiPNet18.10.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet18.10_O2kvsMultiwell.pdf Versions] | |||
|authors=OROBOROS | |||
|year=2014 | |||
|journal=Mitochondr Physiol Network | |||
|abstract='''Gnaiger E, Fasching M (2014) Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(04): 1-8.''' | |||
|keywords=O2k-Specifications | |||
|mipnetlab=AT Innsbruck OROBOROS | |||
}} | |||
{{Labeling | |||
|area=Respiration, Instruments;methods | |||
|organism=Human | |||
|model cell lines=Fibroblast | |||
|preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated Mitochondria, SMP | |||
|instruments=Oxygraph-2k, TIP2k, Fluorometry, pH, NO, TPP, Ca, Theory | |||
}} | |||
» '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]''' | |||
== Discussion == | |||
Scientists who have experience with both instruments are invited to extend the discussion: | |||
::» [[Talk:MiPNet18.10_O2k_versus_multiwell]] | |||
::» [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]] | |||
== Selected related links == | |||
::» [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k] | |||
::» [http://www.oroboros.at/?Power-O2k Power-O2k] | |||
::» [[O2k-Publications: Topics]] | |||
== Conflict of interest == | |||
:: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the Oxygraph-2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations. | |||
== Selected references == | |||
::* [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]] | |||
::* [[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.]] | |||
::* [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]] | |||
::* [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]] | |||
::* [[Huetter 2004 Biochem J|Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]] | |||
::* [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol|Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]] | |||
::* [[Gnaiger 2008 POS|Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]] | |||
::* [[Scandurra 2010 Adv Exp Med Biol|Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.]] | |||
::* [[Aguirre 2010 Biochim Biophys Acta|Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]] | |||
::* [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]] | |||
::* [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] | |||
::* [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]] | |||
{{MitoPedia topics|type=Respiration | |||
}} | |||
[[Category:OroboPedia]] | |||
[[Category:MitoPedia]] | [[Category:MitoPedia]] |
Revision as of 14:45, 8 July 2014
O2k-Specifications
Description | O2k versus multiwell respirometer: O2k stands for Oxygraph-2k and high-resolution respirometry, meeting powerful quality criteria securing high output and pioneering state-of-the-art comprehensive OXPHOS analysis of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. |
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Product ID | |
Type | O2k, Feedback |
Link | Bioblast pdf |
Image |
Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. |
» Open Access-MiPNet18.10.pdf ; Versions
OROBOROS (2014) Mitochondr Physiol Network
Abstract: Gnaiger E, Fasching M (2014) Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(04): 1-8. • Keywords: O2k-Specifications
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Preparation: Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., SMP
HRR: Oxygraph-2k, TIP2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., pH, NO, TPP, Ca, Theory
» More details on O2k-Specifications? Consult OROBOROS
Discussion
Scientists who have experience with both instruments are invited to extend the discussion:
Conflict of interest
- Erich Gnaiger is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the Oxygraph-2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.