Difference between revisions of "O2k-Specifications"

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{{Product
{{Product
|description='''O2k versus multiwell respirometer''': '''O2k''' stands for Oxygraph-2k and '''high-resolution respirometry''', meeting powerful quality criteria securing '''high output''' and pioneering state-of-the-art [[Gnaiger_2012_MitoPathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for '''disposable multiwell''' systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is ''not equivalent to high output''. ''If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target'' ([[Silver 2012 Penguin Press]]).
|description='''O2k versus multiwell respirometer''': '''O2k''' stands for Oxygraph-2k and '''high-resolution respirometry''', meeting powerful quality criteria securing '''high output''' and pioneering state-of-the-art [[Gnaiger_2012_MitoPathways|comprehensive OXPHOS analysis]] of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to '''high output'''. ''If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target'' ([[Silver 2012 Penguin Press]]).
|product id=* [[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access: O2k vs multiwell.pdf''']]
|product id=* [[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access: O2k vs multiwell.pdf''']]
|product type=O2k, Feedback
|product type=O2k, Feedback
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}}
}}
== Discussion ==
== Discussion ==
Scientists who have experience with both instruments are invited to extend the discussion:
:::: Scientists who have experience with both instruments are invited to extend the discussion:
 
::::» [[Microwell respirometry]]
::::» [[Talk:O2k_versus_multiwell_respirometer#Tony_Hickey:_Calibration_is_my_chief_concern |'''Tony Hickey: Calibration is my chief concern''']]
::::» .. and more: [[Talk:O2k versus multiwell respirometer]]
::::» [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]


::» [[Microwell respirometry]]
::» [[Talk:O2k_versus_multiwell_respirometer#Tony_Hickey:_Calibration_is_my_chief_concern |'''Tony Hickey: Calibration is my chief concern''']]
::» .. and more: [[Talk:O2k versus multiwell respirometer]]
::» [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]


== Selected related links ==
== Selected related links ==


::» [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k]  
::::» [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k]  
::» [http://www.oroboros.at/?Power-O2k Power-O2k]
::::» [http://www.oroboros.at/?Power-O2k Power-O2k]
::» [[O2k-Publications: Topics]]
::::» [[O2k-Publications: Topics]]
::» '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]'''
::::» '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]'''




== Conflict of interest ==
== Conflict of interest ==
:: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.
:::: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.
 


== Selected references ==
== Selected references ==


::* Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. [[Gnaiger 1995 J Bioenerg Biomembr |»Bioblast pdf«]]
::::* Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. [[Gnaiger 1995 J Bioenerg Biomembr |»Bioblast pdf«]]
 
::* Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. [[Steinlechner-Maran_1996_Am J Physiol Cell Physiol |»Bioblast pdf«]]


::* Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. [[Gnaiger 2000 Proc Natl Acad Sci U S A |»Open Access«]]
::::* Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. [[Steinlechner-Maran_1996_Am J Physiol Cell Physiol |»Bioblast pdf«]]


::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. [[Gnaiger 2001 Respir Physiol |»Bioblast pdf«]]
::::* Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. [[Gnaiger 2000 Proc Natl Acad Sci U S A |»Open Access«]]


::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. [[Huetter_2004_Biochem J |»Open Access«]]
::::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. [[Gnaiger 2001 Respir Physiol |»Bioblast pdf«]]


::* Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. [[Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol |»Open Access«]]
::::* Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. [[Huetter_2004_Biochem J |»Open Access«]]


::* Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. [[Aragones 2008 Nat Genet |»Open Access«]] </ref>
::::* Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome ''c'' release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. [[Kuznetsov_2004_Am_J_Physiol_Heart_Circ_Physiol |»Open Access«]]


::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast pdf«]]
::::* Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. [[Aragones 2008 Nat Genet |»Open Access«]] </ref>


::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. [[Scandurra 2010 Adv Exp Med Biol |»Bioblast pdf«]]
::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast pdf«]]


::* Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. [[Aguirre 2010 Biochim Biophys Acta ||»Bioblast pdf«]]
::::* Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. [[Scandurra 2010 Adv Exp Med Biol |»Bioblast pdf«]]


::* Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. [[Votion 2012 PLoS One ||»Open Access«]]
::::* Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. [[Aguirre 2010 Biochim Biophys Acta ||»Bioblast pdf«]]


::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast pdf«]]  
::::* Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. [[Votion 2012 PLoS One ||»Open Access«]]


::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Bioblast pdf«]]
::::* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast pdf«]]  


{{MitoPedia topics|type=Respiration
::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Bioblast pdf«]]
}}








{{MitoPedia topics|type=Respiration}}
[[Category:OroboPedia]]
[[Category:OroboPedia]]
[[Category:MitoPedia]]
[[Category:MitoPedia]]

Revision as of 09:26, 21 February 2016



O2k-Specifications

O2k-Catalogue

Description O2k versus multiwell respirometer: O2k stands for Oxygraph-2k and high-resolution respirometry, meeting powerful quality criteria securing high output and pioneering state-of-the-art comprehensive OXPHOS analysis of substrate control and coupling control of mitochondrial function. 'High throughput' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equivalent to high output. If you’re using a biased instrument, it doesn’t matter how many measurements you take – you’re aiming at the wrong target (Silver 2012 Penguin Press).
Product ID
Type O2k, Feedback
Link Bioblast pdf
Image
O2k-Info.png


MitoPedia O2k and high-resolution respirometry: O2k hardware 


Publications in the MiPMap
Logo OROBOROS INSTRUMENTS.jpg
O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. »Bioblast pdf«

» Versions

OROBOROS (2015-05-21) MiPNet

Abstract: Gnaiger E, Fasching M (2015) O2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(05): 1-8. Keywords: O2k-Specifications

O2k-Network Lab: AT Innsbruck OROBOROS


Labels: MiParea: Respiration, Instruments;methods 


Organism: Human 

Preparation: Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP 



HRR: Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory 


Discussion

Scientists who have experience with both instruments are invited to extend the discussion:
» Microwell respirometry
» Tony Hickey: Calibration is my chief concern
» .. and more: Talk:O2k versus multiwell respirometer
» XFe for isolated mitochondria - a critical discussion


Selected related links

» Low running costs for the OROBOROS O2k
» Power-O2k
» O2k-Publications: Topics
» More details on O2k-Specifications? Consult OROBOROS


Conflict of interest

Erich Gnaiger is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the O2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.


Selected references

  • Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. »Bioblast pdf«
  • Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. »Bioblast pdf«
  • Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97:11080-5. »Open Access«
  • Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. »Bioblast pdf«
  • Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. »Open Access«
  • Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286:H1633–41. »Open Access«
  • Aragonés J, Schneider M, Van Geyte K, Fraisl P, Dresselaers T, Mazzone M, Dirkx R, Zacchigna S, Lemieux H, Jeoung NH, Lambrechts D, Bishop T, Lafuste P, Diez-Juan A, K Harten S, Van Noten P, De Bock K, Willam C, Tjwa M, Grosfeld A, Navet R, Moons L, Vandendriessche T, Deroose C, Wijeyekoon B, Nuyts J, Jordan B, Silasi-Mansat R, Lupu F, Dewerchin M, Pugh C, Salmon P, Mortelmans L, Gallez B, Gorus F, Buyse J, Sluse F, Harris RA, Gnaiger E, Hespel P, Van Hecke P, Schuit F, Van Veldhoven P, Ratcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat Genet 40:170-80. »Open Access« </ref>
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast pdf«
  • Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. »Bioblast pdf«
  • Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. |»Bioblast pdf«
  • Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890. |»Open Access«
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast pdf«
  • Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Bioblast pdf«