Difference between revisions of "O2k-Open Support"

Latest revision as of 16:43, 9 August 2022

O2k-Open Support

Currently Asked Questions - »O2k-Open Support alert«

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.

MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
O2k-Manual: topic specific manuals with step by step help
O2k-Videosupport: provides valuable assistance, complementary to the O2k-Manual
O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O₂ sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

Clean O2k-chambers and contamination-free incubation medium are required.
Analysis of DatLab files recorded in instrumental QC tests is essential.
Biological experiments are not suitable for trouble shooting.

1. O₂ sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O₂ sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O₂ sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background test

O2k-Open Support: Installation and startup support session

» MiPNet26.07 Installation and startup support session

Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.

» O2k On-site Training

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.

» Search for defective O2k components

» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support

» O2k-Open Support agreement

» O2k repair

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry

@@ Line 1: / Line 1: @@
-{{OROBOROS header page name}}
+{{Template:OROBOROS support page name}}
-[[Image:O2k-Info.png|left|80px|link=OROBOROS info|OROBOROS info]]
+{{NextGen-O2k H2020-support}}
+[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
-:::: <big><big>We provide service and support</big></big>
+:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>
 __TOC__
-== O2k-Manual and O2k-Protocols ==
+== O2k-Open Support: technical help ==
-'''O2k-technical support''' is based on and extends the guidelines provided with the [[Oxygraph-2k|O2k]]:
-::» [[O2k-Manual]]
-::» [[O2k-Protocols]]
-::» [[O2k-Catalogue OROBOROS |O2k-Catalogue]]
-::» [[O2k-Publications: Topics]]
-Standardized instrumental quality control tests are performed to evaluate and diagnose technical problems fast and cost-effectively.
-::» Polarographic oxygen sensors: calibration, accuracy and quality control SOP. » [[MiPNet06.03 POS-Calibration-SOP]]
-::» Instrumental background correction, accuracy of oxygen flux and SOP. » [[MiPNet14.06 InstrumentalO2Background]]
-::» O2k-standard operating procedures: » [[O2k-SOP]]
-== Open innovation and O2k-technical support ==
-:: The OROBOROS-Team welcomes and values feedback from O2k-users and interested scientists. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, will be relevant to both specialists and beginners in the field.
-<big>'''The OROBOROS-Team offers O2k-technical support based on [[Open innovation management |open innovation]]:'''</big>
-:: Acceptance of O2k-technical support by the OROBOROS-Team implies the mutual agreement to publish the relevant corresponence or a summary of the correspondence addressing technical and scientific questions on O2k-technology and high-resolution respirometry, including names, institutional and Email addresses. Expansion of [[O2k-SOP]]s is particularly relevant for novel approaches in O2k-Fluorometry and O2k-Spectrophotometry, which are combined in the development of the [[NextGen-O2k]]. '''Open innovation''' extends the support given to individual users to the scientific community and provides feedback to improve the scope and documentation of the O2k-technology - in the spirit of [[Gentle Science]].
-== O2k-technical support ==
-=== Application problems ===
-:: An '''application problem''' can be diagnosed on the basis of O2k-calibration and instrumental background tests performed in pure incubation medium in the absence of an experimental sample. Analysis of DatLab files recorded during instrumental tests is essential. If proper instrumental performance is obtained by resolving such application problems, any hardware problem is excluded.
-=== Software problems ===
-:: Communication between the O2k and DatLab running on a computer.
-=== Hardware problems ===
-:: Diagnostic tests are available to identify a defective instrumental component. The two-chamber design of the O2k provides the option to exchange parts between the two chambers and thus locate a defective component: » [[Locating a problem]].
-# Hardware problems that can be solved by user-service: The [[OroboPOS]] (polarographic oxygen sensor) requires user-service at intervals of more than 1 year, and SOPs are available to determine if a sensor service is required at an earlier or later date.
-# Hardware problems that require replacement of a defective part.
-# Some electronic or mechanical defects may be solved only by service of the O2k in the workshop of OROBOROS INSTRUMENTS, e.g. a defective Peltier unit.
-== Cost estimation for repair ==
-:: A cost estimation for repair of the O2k-Main unit due to an  electronic/mechanical defect can only be given after inspection of the  O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customer’s consent. For shipment of the O2k, follow the [[Shipping an oxygraph |detailed instructions]].
-== O2k-technical support and open innovation pages ==
-{{#ask: mainlabel=Title|[[Technical service::+]]
-| ?Technical service
-| limit = 200
-}}
-== We present: [[cell ergometry]] ==
+:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
-[[Image:O2k-Fluorometer.JPG|120px|left|link=O2k-Fluorometer|O2k-Fluorometer]]
+::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
-::* '''»''' Explore cell respiration and key mitochondrial functions''': [[High-resolution respirometry |» HRR]] and the [http://www.oroboros.at/?Oxygraph » O2k].
+::::::## '''[[MitoPedia: O2k-Open Support]]'''
-::* '''»''' A concept complete with elaborated protocols, to get started and gain competence in the expanding field of bioenergetics: » [[Gnaiger 2014 MitoPathways |Blue Book 2014]] - » [[O2k-Protocols]].
+::::::## [[MitoPedia: O2k hardware]]
-::* '''»''' Introductory courses and advanced training: » [[OROBOROS_Events]].
+::::::## [[MitoPedia: DatLab]]
-::'''»'''  [http://www.oroboros.at/?Purchase WorldWide distribution and customer support].
+::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
+::::::# [[O2k-Manual]]: topic specific manuals with step by step help
+::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
+::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
-<br />
-<br />
-== Training courses ==
+::: '''<big>O2k-Quality Control</big>'''
+[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
+:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].
-[[Image:O2k-Workshops.png|left|120px|link=OROBOROS_Events|O2k-Workshops]]
+:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
+::::# Clean O2k-chambers and contamination-free incubation medium are required.
+::::# Analysis of DatLab files recorded in instrumental QC tests is essential.
+::::# Biological experiments are not suitable for trouble shooting.
-:: Join us at '''[[OROBOROS_Events|O2k-Workshops]]''' in the fast lane to high-resolution respirometry.
+=== 1. O<sub>2</sub> sensor test ===
+:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
+::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]
+=== 2. O2k-chamber test ===
+:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
+::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]
-== OROBOROS Mitochondrial Research Laboratory ==
+== O2k-Open Support: Installation and startup support session ==
+:::::» '''[[MiPNet26.07 Installation and startup support session]]'''
-:: The [[OROBOROS Mitochondrial Research Laboratory |'''OROBOROS MitoLab''']] is open for innovation and cooperation.
+:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
+:::::» '''[[O2k On-site Training]]'''
-== The O2k-Network - are you connected? ==
+[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
+== O2k-Open Support agreement ==
-:: The OROBOROS O2k-Network serves to connect and support:
+:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
+::::» [[Search for defective O2k components]]
+:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']
+::::» [[O2k-Open Support agreement]]
+::::» [[O2k repair]]
-[[Image:O2k-Network.png|left|120px|link=O2k-Network|O2k-Network]]
+[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
-::* '''»''' The O2k-Network Labs: [[O2k-Network |» O2k-Network]].
+== The O2k-Network ==
-::* '''»''' Communication on a scientific level: [[O2k versus multiwell respirometer]]
-::* '''»''' Studies in mitochondrial physiology: [http://www.oroboros.at/?high-resolution-respirometry » Applications].
-::* '''»''' [[OROROBOS Science Scholarship]]s
-::* '''»''' Scientific expeditions: [http://www.oroboros.at/?Expeditions » Science and adventure].
-<br />
+:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
+::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]
-== Contact ==
+::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]
-:: In case you need further technical support, contact [mailto:[email protected]| [email protected]]
+:::: '''»''' [[Oroboros Laboratory|Oroboros O2k-Laboratory]] - open for innovation and cooperation.
+:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry
-[[Category:OroboPedia]]
+[[Category:O2k-Open Support]]