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Nystroem 1996 EMBO J

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Nyström T, Larsson C, Gustafsson L (1996) Bacterial defence against ageing: Role of the Escherichia coli ArcA regulator in gene expression, readjusted energy flux and survival during stress. EMBO J 15:3219-28.

» PMID: 8670822 Open Access

Nystroem T, Larsson C, Gustafsson L (1996) EMBO J

Abstract: Using two-dimensional gel electrophoresis and Nterminal amino acid sequencing analysis, we demonstrate that a mutant of the global regulatory protein ArcA fails to decrease the synthesis of the TCA cycle enzymes malate dehydrogenase, isocitrate dehydrogenase, lipoamide dehydrogenase E3 and succinate dehydrogenase in response to stasis, while the increased production of the glycolysis enzymes phosphoglycerate mutase and pyruvate kinase is unaffected. Microcalorimetric and respiratory measurements show that the continued production of TCA cycle enzymes in the AarcA mutant is manifested as an elevated rate of respiration and total metabolic activity during starvation. The AarcA mutant is severely impaired in surviving prolonged periods of exogenous carbon starvation, a phenotype that can be alleviated by overproducing the superoxide dismutase SodA. In addition, flow cytometry demonstrates that starving AarcA mutant cells, in contrast to wild-type cells, fail to perform reductive division, remain large and contain multiple chromosomal copies. We suggest that the ArcA-dependent reduced production of electron donors and the decreased level and activity of the aerobic respiratory apparatus during growth arrest is an integral part of a defense system aimed at avoiding the damaging effects of oxygen radicals and controlling the rate of utilization of endogenous reserves. Keywords: Aging, ArcA, Escherichia coli, Glucose starvation stimulon, SodA

O2k-Network Lab: SE Gothenburg Gustafsson L

Labels: Pathology: Aging;senescence 

Enzyme: TCA cycle and matrix dehydrogenases  Regulation: Substrate  Coupling state: OXPHOS 

HRR: Oxygraph-2k