Difference between revisions of "Noncoupled respiration"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''E'' | |abbr=''E'' | ||
|description='''Noncoupled respiration''' is distinguished from general (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the | |description='''Noncoupled respiration''' is distinguished from general (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of [[ETS]] capacity. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above [[optimum uncoupler concentration]]s or insufficient stimulation by sub-optimal uncoupler concentrations. '''[[Optimum uncoupler concentration]]s''' for evaluation of (noncoupled) ETS capacity require inhibitor titrations ([[Steinlechner-Maran_1996_AJP]]; [[Huetter_2004_BJ]]; [[Gnaiger_2008_POS]]). | ||
Noncoupled respiration is maximum [[electron flow]] in an open-transmembrane proton circuit mode of operation (see [[ETS capacity]]). | Noncoupled respiration is maximum [[electron flow]] in an open-transmembrane proton circuit mode of operation (see [[ETS capacity]]). | ||
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== Biochemical coupling efficiency: from 0 to <1 == | == Biochemical coupling efficiency: from 0 to <1 == | ||
* ''More details:'' [[Biochemical coupling efficiency]] | * ''More details:'' [[Biochemical coupling efficiency]] | ||
== Is respiration uncoupled - noncoupled - dyscoupled? == | |||
* ''More details:'' [[Uncoupler]] | |||
Revision as of 18:33, 20 April 2014
- high-resolution terminology - matching measurements at high-resolution
Noncoupled respiration
Description
Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ETS capacity. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ETS capacity require inhibitor titrations (Steinlechner-Maran_1996_AJP; Huetter_2004_BJ; Gnaiger_2008_POS).
Noncoupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ETS capacity).
Abbreviation: E
Reference: Gnaiger 2012 MitoPathways, MitoPedia Glossary: Respiratory states
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Biochemical coupling efficiency: from 0 to <1
- More details: Biochemical coupling efficiency
Is respiration uncoupled - noncoupled - dyscoupled?
- More details: Uncoupler
