Difference between revisions of "Noncoupled respiration"

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high-resolution terminology - matching measurements at high-resolution

Noncoupled respiration

Description

Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ETS capacity. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ETS capacity require inhibitor titrations (Steinlechner-Maran 1996 Am J Physiol Cell Physiol; Huetter 2004 Biochem J; Gnaiger 2008 POS).

Noncoupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ETS capacity).

Abbreviation: E

Reference: Gnaiger 2014 MitoPathways, MitoPedia: Respiratory states

MitoPedia concepts: Respiratory state, Recommended

MitoPedia methods: Respirometry

MitoPedia topics: EAGLE

Contributed by Gnaiger E 2010-08-15, edited 2015-01-19.

Is respiration uncoupled - noncoupled - dyscoupled?

» More details: Uncoupler

Biochemical coupling efficiency: from 0 to <1

» More details: OXPHOS coupling efficiency and ETS coupling efficiency

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 {{MitoPedia
 |abbr=''E''
-|description=[[File:E.jpg |link=ETS capacity]] '''Noncoupled respiration''' is distinguished from general (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of [[ETS]] capacity. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above [[optimum uncoupler concentration]]s or insufficient stimulation by sub-optimal uncoupler concentrations. '''[[Optimum uncoupler concentration]]s''' for evaluation of (noncoupled) ETS capacity require inhibitor titrations ([[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]; [[Huetter 2004 Biochem J]]; [[Gnaiger 2008 POS]]).
+|description=[[File:E.jpg |link=ETS capacity]] '''Noncoupled respiration''' is distinguished from general (pharmacological or mechanical) [[uncoupled respiration]], to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of [[ETS capacity]]. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above [[optimum uncoupler concentration]]s or insufficient stimulation by sub-optimal uncoupler concentrations. '''[[Optimum uncoupler concentration]]s''' for evaluation of (noncoupled) ETS capacity require inhibitor titrations ([[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]]; [[Huetter 2004 Biochem J]]; [[Gnaiger 2008 POS]]).
 Noncoupled respiration is maximum [[electron flow]] in an open-transmembrane proton circuit mode of operation (see [[ETS capacity]]).