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NADH

From Bioblast


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NADH

Description

Nicotinadeninedinucleotide (NADH) is not permeable through the inner mt-membrane. The NADH pool integrates the activity of the TCA cycle and various matrix dehydrogenases upstream of CI, and thus forms a junction or funnel of electron transfer to CI. Cytosolic NADH is effectively made available for mitochondrial respiration through the malate-aspartate shuttle or glycerophosphate dehydrogenase.

Abbreviation: n, NADH



MitoPedia topics: Substrate and metabolite 


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Enzyme: Complex I 





Application in HRR

n: Nicotinadeninedinucleotide, NADH (Ξ²-Nicotinamide adenine dinucleotide, reduced potassium salt, C21H27N7O14P2K2); Sigma A 4505, 100 mg, store at -20 Β°C (old Sigma recommendation : 4-8Β°); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [1]. The same precautions are recommended by Sigma for the sodium salt.