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Difference between revisions of "NADH"

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{{MitoPedia
{{MitoPedia
|abbr=NADH
|abbr=NADH
|description='''Nicotinadeninedinucleotide (NADH)''' is not permeable through the inner mt-membrane.Β  The NADH pool integrates the activity of the [[TCA cycle]] and various matrix dehydrogenases upstream of [[CI]], and thus forms a junction or funnel of electron transfer to CI.Β  Cytosolic NADH is effectively made available for mitochondrial respiration through the [[malate-aspartate shuttle]] or [[glycerophosphate dehydrogenase]].
|description='''NAD<sup>+</sup>''' and '''NADH''': see [[Nicotinamide adenine dinucleotide]].
|type=Substrate ETS
}}
{{MitoPedia methods|type=Substrate ETS
}}
}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
|mitopedia topic=Substrate and metabolite
|type=Substrate ETS
}}
{{Labeling
|enzymes=TCA Cycle and Matrix Dehydrogenases, Complex I, Marker Enzyme
|type=Substrate ETS
}}
}}
== Application in [[HRR]]Β  ==
'''NADH: Nicotinadeninedinucleotide''' (Ξ²-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>);Β  Sigma A 4505, 100 mg, store at -20 Β°C (old Sigma recommendation : 4-8Β°); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt.

Revision as of 05:00, 12 February 2016


high-resolution terminology - matching measurements at high-resolution


NADH

Description

NAD+ and NADH: see Nicotinamide adenine dinucleotide.

Abbreviation: NADH






MitoPedia topics: Substrate and metabolite