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| {{MitoPedia | | {{MitoPedia |
| |abbr=NADH | | |abbr=NADH |
| |description='''Nicotinadeninedinucleotide (NADH)''' is not permeable through the inner mt-membrane.Β The NADH pool integrates the activity of the [[TCA cycle]] and various matrix dehydrogenases upstream of [[CI]], and thus forms a junction or funnel of electron transfer to CI.Β Cytosolic NADH is effectively made available for mitochondrial respiration through the [[malate-aspartate shuttle]] or [[Glycerophosphate_dehydrogenase_complex|glycerophosphate dehydrogenase]]. | | |description='''NAD<sup>+</sup>''' and '''NADH''': see [[Nicotinamide adenine dinucleotide]]. |
| |type=Substrate ETS
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| {{MitoPedia methods|type=Substrate ETS
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| | {{MitoPedia O2k and high-resolution respirometry}} |
| {{MitoPedia topics | | {{MitoPedia topics |
| |mitopedia topic=Substrate and metabolite | | |mitopedia topic=Substrate and metabolite |
| |type=Substrate ETS
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| {{Labeling
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| |enzymes=Complex I
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| == Application in [[HRR]]Β ==
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| '''n: Nicotinadeninedinucleotide, NADH''' (Ξ²-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>);Β Sigma A 4505, 100 mg, store at -20 Β°C (old Sigma recommendation : 4-8Β°); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt.
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Revision as of 06:00, 12 February 2016
