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Difference between revisions of "N-junction"

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{{MitoPedia
{{MitoPedia
|description=[[File:SUIT-Catg N.jpg|right|300px|N-junction]]
|description=[[File:SUIT-catg N.jpg|right|300px|N-junction]]
The '''N-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer system]] (ETS) from [[NADH-linked substrate state |type N substrates]] through the mt-[[NADH]] pool to [[Complex I]] (CI), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). Representative [[Substrate types |type N substrates]] are pyruvate, glutamate and malate. The corresponding dehydrogenases (PDH, GDH, MDH) generate NADH, the substrate of [[Complex I]] (CI). The concept of the N-junction and [[F-junction]] provides a basis for defining [[categories of SUIT protocols]] based on [[substrate types]].
The '''N-junction''' is a junction for [[convergent electron flow]] in the [[electron transfer pathway]] (ET-pathway) from type N substrates (''further details'' Β»[[N-pathway control state]]) through the mt-[[NADH]] pool to [[Complex I]] (CI), and further transfer through the [[Q-junction]] to [[Complex III]] (CIII). Representative type N substrates are pyruvate (P), glutamate (G) and malate (M). The corresponding dehydrogenases ([[Pyruvate dehydrogenase |PDH]], [[Glutamate dehydrogenase |GDH]], [[Malate dehydrogenase |MDH]]) and some additional TCA cycle dehydrogenases ([[isocitrate dehydrogenase]], [[oxoglutarate dehydrogenase]] generate NADH, the substrate of [[Complex I]] (CI). The concept of the N-junction and [[F-junction]] provides a basis for defining [[categories of SUIT protocols]] based on [[Electron-transfer-pathway state]]s.
|info=[[Gnaiger 2014 MitoPathways]]
|info=[[N-pathway control state]]
}}
{{MitoPedia concepts
|mitopedia concept=MiP concept, SUIT concept
}}
}}
{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry
|mitopedia method=Respirometry
}}
}}
{{MitoPedia O2k and high-resolution respirometry
Β  Contributed by [[Gnaiger E]] 2016-02-12; edited 2016-08-28, 2017-01-03.
|mitopedia O2k and high-resolution respirometry=SUIT protocol
}}
Β  Contributed by [[Gnaiger E]] 2016-02-12

Latest revision as of 13:20, 8 June 2020


high-resolution terminology - matching measurements at high-resolution


N-junction

Description

N-junction

The N-junction is a junction for convergent electron flow in the electron transfer pathway (ET-pathway) from type N substrates (further details Β»N-pathway control state) through the mt-NADH pool to Complex I (CI), and further transfer through the Q-junction to Complex III (CIII). Representative type N substrates are pyruvate (P), glutamate (G) and malate (M). The corresponding dehydrogenases (PDH, GDH, MDH) and some additional TCA cycle dehydrogenases (isocitrate dehydrogenase, oxoglutarate dehydrogenase generate NADH, the substrate of Complex I (CI). The concept of the N-junction and F-junction provides a basis for defining categories of SUIT protocols based on Electron-transfer-pathway states.


Reference: N-pathway control state


MitoPedia concepts: MiP concept, SUIT concept 


MitoPedia methods: Respirometry 

Contributed by Gnaiger E 2016-02-12; edited 2016-08-28, 2017-01-03.