Murphy 1997 Exp Parasitol
| Murphy AD, Doeller JE, Lang-Unnasch N (1997) Plasmodium falciparum: Cyanide-resistant oxygen consumption. Exp. Parasitol. 87: 112-120. |
Murphy AD, Doeller JE, Lang-Unnasch N (1997) Experimental Parasitology
Abstract: It has been hypothesized that Plasmodiumparasites utilize a branched chain respiratory pathway, consisting of a classical cyanide-sensitive branch and an alternative cyanide-resistant branch. To further explore this hypothesis, the effect of cyanide on Plasmodium falciparumwas determined using a polarographic assay. The rate of oxygen consumption by saponin-freed parasites was approximately 5% that of control human white blood cells or of Toxoplasma gondii,consistent with an anabolic role for P. falciparumrespiration. However, while all of the oxygen consumption of the control white blood cells and of T. gondiicould be inhibited by cyanide, 25% of the oxygen consumption of theP. falciparumparasites was found to be insensitive to high concentrations of cyanide. The cyanide-resistant portion of the parasite oxygen consumption was completely inhibited by two inhibitors of alternative oxidase activities in other systems, propyl gallate and salicyclhydroxamic acid. These studies provide the first direct evidence for a branched chain respiratory pathway inP. falciparum.Furthermore, salicyclhydroxamic acid, propyl gallate, and related inhibitors of alternative oxidase activities were shown to inhibit the growth ofP. falciparum in vitro.These results support the need for further investigation of alternative oxidase activity as an antimalarial chemotherapeutic target. • Keywords: Alternative respiration pathway, Branched respiratory chain, Classical respiration pathway, Malaria, Mitochondrial electron transport, Plasmodium falciparum, Propyl gallate
Labels:
Organism: Bacteria"Bacteria" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
