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Difference between revisions of "Mozo 2006 Biochem J"

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{{Publication
{{Publication
|title=Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Expression of UCP3 in CHO cells does not cause uncoupling but controls mitochondrial activity in the presence of glucose. Biochem. J. 393: 431-439.
|title=Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Expression of UCP3 in CHO cells does not cause uncoupling but controls mitochondrial activity in the presence of glucose. Biochem J 393:431-9.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16178820 PMID: 16178820]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16178820 PMID: 16178820 Open Access]
|authors=Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F
|authors=Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F
|year=2006
|year=2006
|journal=Biochem. J.
|journal=Biochem J
|abstract=The proton-transport activity of UCP1 (uncoupling protein 1)
|abstract=The proton-transport activity of UCP1 (uncoupling protein 1)
triggers mitochondrial uncoupling and thermogenesis. The exact
triggers mitochondrial uncoupling and thermogenesis. The exact
role of its close homologues, UCP2 and UCP3, is unclear.Mounting
role of its close homologues, UCP2 and UCP3, is unclear.Mounting
evidence associates them with the control of mitochondrial
evidence associates them with the control of mitochondrial
superoxide production. UsingCHO(Chinese-hamster ovary) cells
superoxide production. Using CHO(Chinese-hamster ovary) cells
stably expressing UCP3 or UCP1, we found no evidence for respiration
stably expressing UCP3 or UCP1, we found no evidence for respiration
uncoupling. The explanation lies in the absence of an
uncoupling. The explanation lies in the absence of an
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UCP1.
UCP1.
|keywords=Glycolysis, Membrane potential, Mitochondria, Pyruvate, Reactive oxygen species, Uncoupling protein (UCP)
|keywords=Glycolysis, Membrane potential, Mitochondria, Pyruvate, Reactive oxygen species, Uncoupling protein (UCP)
|mipnetlab=FR_Paris_Bouillaud F
|mipnetlab=FR Paris Bouillaud F
}}
}}
{{Labeling
{{Labeling
|area=Respiration
|organism=Other mammals
|tissues=CHO
|preparations=Intact cells
|topics=ADP, Substrate, Temperature, Uncoupler
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|organism=Other Mammal
|preparations=Intact Cell; Cultured; Primary
|kinetics=ADP; Pi, Inhibitor; Uncoupler, Temperature
|topics=Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential, Substrate; Glucose; TCA Cycle
}}
}}

Revision as of 09:27, 9 November 2016

Publications in the MiPMap
Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Expression of UCP3 in CHO cells does not cause uncoupling but controls mitochondrial activity in the presence of glucose. Biochem J 393:431-9.

Β» PMID: 16178820 Open Access

Mozo J, Ferry G, Studeny A, Pecqueur C, Rodriguez M, Boutin JA, Bouillaud F (2006) Biochem J

Abstract: The proton-transport activity of UCP1 (uncoupling protein 1) triggers mitochondrial uncoupling and thermogenesis. The exact role of its close homologues, UCP2 and UCP3, is unclear.Mounting evidence associates them with the control of mitochondrial superoxide production. Using CHO(Chinese-hamster ovary) cells stably expressing UCP3 or UCP1, we found no evidence for respiration uncoupling. The explanation lies in the absence of an appropriate activator of UCP protonophoric function. Accordingly, the addition of retinoic acid uncouples the respiration of the UCP1-expressing clone, but not that of the UCP3-expressing ones. In a glucose-containing medium, the extent of the hyperpolarization of mitochondria by oligomycin was close to 22 mV in the five UCP3-expressing clones, contrasting with the variable values observed with the 15 controls. Our observations suggest that, when glycolysis and mitochondria generate ATP, and in the absence of appropriate activators of proton transport, UCPs do not transport protons (uncoupling), but rather other ions of physiological relevance that control mitochondrial activity. A model is proposed using the known passive transport of pyruvate by UCP1. β€’ Keywords: Glycolysis, Membrane potential, Mitochondria, Pyruvate, Reactive oxygen species, Uncoupling protein (UCP)

β€’ O2k-Network Lab: FR Paris Bouillaud F


Labels: MiParea: Respiration 


Organism: Other mammals  Tissue;cell: CHO  Preparation: Intact cells 

Regulation: ADP, Substrate, Temperature, Uncoupler 


HRR: Oxygraph-2k