Difference between revisions of "Montero 2015 J Physiol"

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{{Publication
{{Publication
|title=Montero D, Cathomen A, Jacobs RA, Flück D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C (2015) Haematological rather than skeletal muscle adaptations contribute to the increase in peak oxygen uptake induced by moderate endurance training. J Physiol [Epub ahead of print].  
|title=Montero D, Cathomen A, Jacobs RA, Flück D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C (2015) Haematological rather than skeletal muscle adaptations contribute to the increase in peak oxygen uptake induced by moderate endurance training. J Physiol 593:4677-88.
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26282186 PMID: 26282186]
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26282186 PMID: 26282186]
|authors=Montero D, Cathomen A, Jacobs RA, Flück D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C
|authors=Montero D, Cathomen A, Jacobs RA, Flueck D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C
|year=2015
|year=2015
|journal=J Physiol
|journal=J Physiol
|abstract=KEY POINTS:
|abstract=KEY POINTS:
This study assessed the respective contributions of haematological and skeletal muscle adaptations to any observed improvement in peak oxygen uptake (V̇O2 peak ) induced by endurance training (ET). V̇O2 peak , peak cardiac output (Q̇ peak ), blood volumes and skeletal muscle biopsies were assessed prior (pre) to and after (post) 6 weeks of ET. Following the post-ET assessment, red blood cell volume (RBCV) reverted to the pre-ET level following phlebotomy and V̇O2 peak and Q̇ peak were determined again. We speculated that the contribution of skeletal muscle adaptations to an ET-induced increase in V̇O2 peak could be identified when offsetting the ET-induced increase in RBCV. V̇O2 peak , Q̇ peak , blood volumes, skeletal muscle mitochondrial volume density and capillarization were increased after ET. Following RBCV normalization, V̇O2 peak and Q̇ peak reverted to pre-ET levels. These results demonstrate the predominant contribution of haematological adaptations to any increase in V̇O2 peak induced by ET.
This study assessed the respective contributions of haematological and skeletal muscle adaptations to any observed improvement in peak oxygen uptake (VO<sub>2</sub> peak ) induced by endurance training (ET). VO<sub>2</sub> peak , peak cardiac output (Q̇ peak ), blood volumes and skeletal muscle biopsies were assessed prior (pre) to and after (post) 6 weeks of ET. Following the post-ET assessment, red blood cell volume (RBCV) reverted to the pre-ET level following phlebotomy and VO<sub>2</sub> peak and Q̇ peak were determined again. We speculated that the contribution of skeletal muscle adaptations to an ET-induced increase in VO<sub>2</sub> peak could be identified when offsetting the ET-induced increase in RBCV. VO<sub>2</sub> peak , Q̇ peak , blood volumes, skeletal muscle mitochondrial volume density and capillarization were increased after ET. Following RBCV normalization, VO<sub>2</sub> peak and Q̇ peak reverted to pre-ET levels. These results demonstrate the predominant contribution of haematological adaptations to any increase in VO<sub>2</sub> peak induced by ET.


ABSTRACT:
ABSTRACT:
It remains unclear whether improvements in peak oxygen uptake (V̇O2 peak ) following endurance training (ET) are primarily determined by central and/or peripheral adaptations. Herein, we tested the hypothesis that the improvement in V̇O2 peak following 6 weeks of ET is mainly determined by haematological rather than skeletal muscle adaptations. Sixteen untrained healthy male volunteers (age = 25 ± 4 years, V̇O2 peak  = 3.5 ± 0.5 l min-1 ) underwent supervised ET (6 weeks, 3-4 sessions per week). V̇O2 peak , peak cardiac output (Q̇ peak ), haemoglobin mass (Hbmass ) and blood volumes were assessed prior to and following ET. Skeletal muscle biopsies were analysed for mitochondrial volume density (MitoVD ), capillarity, fibre types and respiratory capacity (OXPHOS). After the post-ET assessment, red blood cell volume (RBCV) was re-established at the pre-ET level by phlebotomy and V̇O2 peak and Q̇ peak were measured again. We speculated that the contribution of skeletal muscle adaptations to the ET-induced increase in V̇O2 peak would be revealed when controlling for haematological adaptations. V̇O2 peak and Q̇ peak were increased (P < 0.05) following ET (9 ± 8 and 7 ± 6%, respectively) and decreased (P < 0.05) after phlebotomy (-7 ± 7 and -10 ± 7%). RBCV, plasma volume and Hbmass all increased (P < 0.05) after ET (8 ± 4, 4 ± 6 and 6 ± 5%). As for skeletal muscle adaptations, capillary-to-fibre ratio and total MitoVD increased (P < 0.05) following ET (18 ± 16 and 43 ± 30%), but OXPHOS remained unaltered. Through stepwise multiple regression analysis, Q̇ peak , RBCV and Hbmass were found to be independent predictors of V̇O2 peak . In conclusion, the improvement in V̇O2 peak following 6 weeks of ET is primarily attributed to increases in Q̇ peak and oxygen-carrying capacity of blood in untrained healthy young subjects.
It remains unclear whether improvements in peak oxygen uptake (V̇O2 peak ) following endurance training (ET) are primarily determined by central and/or peripheral adaptations. Herein, we tested the hypothesis that the improvement in V̇O2 peak following 6 weeks of ET is mainly determined by haematological rather than skeletal muscle adaptations. Sixteen untrained healthy male volunteers (age = 25 ± 4 years, V̇O2 peak  = 3.5 ± 0.5 l min-1 ) underwent supervised ET (6 weeks, 3-4 sessions per week). V̇O2 peak , peak cardiac output (Q̇ peak ), haemoglobin mass (Hbmass ) and blood volumes were assessed prior to and following ET. Skeletal muscle biopsies were analysed for mitochondrial volume density (MitoVD ), capillarity, fibre types and respiratory capacity (OXPHOS). After the post-ET assessment, red blood cell volume (RBCV) was re-established at the pre-ET level by phlebotomy and V̇O2 peak and Q̇ peak were measured again. We speculated that the contribution of skeletal muscle adaptations to the ET-induced increase in V̇O2 peak would be revealed when controlling for haematological adaptations. V̇O2 peak and Q̇ peak were increased (P < 0.05) following ET (9 ± 8 and 7 ± 6%, respectively) and decreased (P < 0.05) after phlebotomy (-7 ± 7 and -10 ± 7%). RBCV, plasma volume and Hbmass all increased (P < 0.05) after ET (8 ± 4, 4 ± 6 and 6 ± 5%). As for skeletal muscle adaptations, capillary-to-fibre ratio and total MitoVD increased (P < 0.05) following ET (18 ± 16 and 43 ± 30%), but OXPHOS remained unaltered. Through stepwise multiple regression analysis, Q̇ peak , RBCV and Hbmass were found to be independent predictors of V̇O2 peak . In conclusion, the improvement in V̇O2 peak following 6 weeks of ET is primarily attributed to increases in Q̇ peak and oxygen-carrying capacity of blood in untrained healthy young subjects.
|mipnetlab=CH Zurich Gassmann M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology
|mipnetlab=CH Zurich Gassmann M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, US CO Colorado Springs Jacobs RA
}}
}}
{{Labeling
{{Labeling
Line 17: Line 17:
|tissues=Skeletal muscle
|tissues=Skeletal muscle
|preparations=Permeabilized tissue
|preparations=Permeabilized tissue
|couplingstates=OXPHOS, ETS
|couplingstates=OXPHOS, ET
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
}}
}}

Latest revision as of 12:04, 28 March 2018

Publications in the MiPMap
Montero D, Cathomen A, Jacobs RA, Flück D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C (2015) Haematological rather than skeletal muscle adaptations contribute to the increase in peak oxygen uptake induced by moderate endurance training. J Physiol 593:4677-88.

» PMID: 26282186

Montero D, Cathomen A, Jacobs RA, Flueck D, de Leur J, Keiser S, Bonne T, Kirk N, Lundby AK, Lundby C (2015) J Physiol

Abstract: KEY POINTS: This study assessed the respective contributions of haematological and skeletal muscle adaptations to any observed improvement in peak oxygen uptake (VO2 peak ) induced by endurance training (ET). VO2 peak , peak cardiac output (Q̇ peak ), blood volumes and skeletal muscle biopsies were assessed prior (pre) to and after (post) 6 weeks of ET. Following the post-ET assessment, red blood cell volume (RBCV) reverted to the pre-ET level following phlebotomy and VO2 peak and Q̇ peak were determined again. We speculated that the contribution of skeletal muscle adaptations to an ET-induced increase in VO2 peak could be identified when offsetting the ET-induced increase in RBCV. VO2 peak , Q̇ peak , blood volumes, skeletal muscle mitochondrial volume density and capillarization were increased after ET. Following RBCV normalization, VO2 peak and Q̇ peak reverted to pre-ET levels. These results demonstrate the predominant contribution of haematological adaptations to any increase in VO2 peak induced by ET.

ABSTRACT: It remains unclear whether improvements in peak oxygen uptake (V̇O2 peak ) following endurance training (ET) are primarily determined by central and/or peripheral adaptations. Herein, we tested the hypothesis that the improvement in V̇O2 peak following 6 weeks of ET is mainly determined by haematological rather than skeletal muscle adaptations. Sixteen untrained healthy male volunteers (age = 25 ± 4 years, V̇O2 peak = 3.5 ± 0.5 l min-1 ) underwent supervised ET (6 weeks, 3-4 sessions per week). V̇O2 peak , peak cardiac output (Q̇ peak ), haemoglobin mass (Hbmass ) and blood volumes were assessed prior to and following ET. Skeletal muscle biopsies were analysed for mitochondrial volume density (MitoVD ), capillarity, fibre types and respiratory capacity (OXPHOS). After the post-ET assessment, red blood cell volume (RBCV) was re-established at the pre-ET level by phlebotomy and V̇O2 peak and Q̇ peak were measured again. We speculated that the contribution of skeletal muscle adaptations to the ET-induced increase in V̇O2 peak would be revealed when controlling for haematological adaptations. V̇O2 peak and Q̇ peak were increased (P < 0.05) following ET (9 ± 8 and 7 ± 6%, respectively) and decreased (P < 0.05) after phlebotomy (-7 ± 7 and -10 ± 7%). RBCV, plasma volume and Hbmass all increased (P < 0.05) after ET (8 ± 4, 4 ± 6 and 6 ± 5%). As for skeletal muscle adaptations, capillary-to-fibre ratio and total MitoVD increased (P < 0.05) following ET (18 ± 16 and 43 ± 30%), but OXPHOS remained unaltered. Through stepwise multiple regression analysis, Q̇ peak , RBCV and Hbmass were found to be independent predictors of V̇O2 peak . In conclusion, the improvement in V̇O2 peak following 6 weeks of ET is primarily attributed to increases in Q̇ peak and oxygen-carrying capacity of blood in untrained healthy young subjects.


O2k-Network Lab: CH Zurich Gassmann M, CH Zurich Lundby C, CH Zurich University of Zurich Physiology, US CO Colorado Springs Jacobs RA


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: OXPHOS, ET 

HRR: Oxygraph-2k