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Difference between revisions of "MitoKit-CII/Succinate-nv"

From Bioblast
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== Application in HRR ==
== Application in HRR ==
:::: '''Snv: NV118''' 25 mg, MW=262.2 g.mol-1
:::: '''Snv: NV118''' 25 mg, MW=262.2 g.mol<sup>-1</sup>
:::: '''Preparation of 1 M stock solution:'''
:::: '''Preparation of 1 M stock solution:'''
::::# Dissolve 25 mg NV118 in 95.1 µL DMSO.
::::# Dissolve 25 mg NV118 in 95.1 µL DMSO.
Line 17: Line 17:
::::# Store at -20 °C.
::::# Store at -20 °C.


::: '''O2k manual titrations'''  
:::» '''O2k manual titrations'''  
::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
::::* Final concentration: 50 µM steps
::::* Final concentration: 50 µM steps
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.
::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.

Revision as of 14:12, 11 April 2019


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII/Succinate-nv

Description

NV118/MitoKit-CII (diacetoxymethyl succinate) is a plasma membrane-permeable (p) prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing succinate in the intracellular space.

Abbreviation: Snv

Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun


MitoPedia topics: Media for respirometry 


Application in HRR

Snv: NV118 25 mg, MW=262.2 g.mol-1
Preparation of 1 M stock solution:
  1. Dissolve 25 mg NV118 in 95.1 µL DMSO.
Preparation of 100 mM stock solution (from the 1M stock):
  1. Dilute 95.1 µL 1 M stock solution with 855.9 µL DMSO.
  2. Divide into 50 µL portions.
  3. Store at -20 °C.
» O2k manual titrations
  • Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
  • Final concentration: 50 µM steps
  • Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.